C3对称性含膦三足体衍生物及其Eu(III)配合物的合成以及与DNA相互作用的研究  

Synthesis and the Interaction with DNA of C3 Symmetry Phosphorus-Containing Tripod Ligand and Its Eu(III) Complex

作　　者：杨水兰[1] 宋盼[1] 佘文洁 杨天林[1] 

机构地区：[1]宁夏大学化学化工学院能源重点实验室,银川750021

出　　处：《化学通报》2015年第5期438-446,共9页Chemistry

基　　金：国家自然科学基金项目(21261018);教育部科学基金重点项目(208195)资助

摘　　要：设计合成了一种新的C3对称性含膦三足体衍生物N',N″,N-三(亚磷酸三乙酯)缩氨三乙酸(L)及其Eu(Ⅲ)配合物。用1H NMR、13C NMR、红外光谱、元素分析、差热-热重及紫外光谱对其组成和结构进行分析和表征。结果表明,三足体衍生物与稀土苦味酸盐(Eu(pic)3·6H2O)形成了1∶1配合物Eu(pic)3L。综合运用紫外-可见吸收光谱法、荧光光谱法和循环伏安法研究了Eu(pic)3L与小牛胸腺DNA之间的结合模式,结果表明,配合物Eu(pic)3L与DNA之间以嵌插形式发生相互作用。将该配合物作为杂交探针,对其在DNA电化学传感器方面的应用进行了探讨。结果发现,该配合物在修饰单链DNA的电极检测作用下,无明显的电化学信号响应,而当将其用于检测杂交双链DNA时,出现了明显信号,并且该配合物的DNA传感器对互补序列、错配序列及非互补序列都有良好的选择作用。A novel C3 symmetry tripod ligand N',N″,N-tri(triethyl phosphite) shrink nitrilotriacetic acid(L)and its Eu(Ⅲ) complex Eu(pic)3L were synthesized and characterized by elemental analysis,IR,NMR,DSC,and TGA. The results of UV-Vis,fluorescent spectra and CV indicated that there are intercalation interaction between calf thymus DNA and Eu(pic)3L. The study on electrochemical sensor performance of Eu(pic)3L showed that there was no obvious electrochemical response for the single-strand DNA,while the obvious electrochemical response was investigated for the double-stranded DNA. So Eu(pic)3L could be well used as the biosensor to discriminate the complementary sequences from the base-mismatched and the non-complementary sequences.

关 键 词：含膦三足体 稀土苦味酸盐配合物 结合模式 杂交探针 

分 类 号：O641.4[理学—物理化学]