豚鼠脑缺血再灌注损伤后内耳凋亡诱导因子的表达及与细胞凋亡的关系  

Relationship between apoptosis and expression of apoptosis inducing factor in cochlear after cerebral ischemia-reperfusion injury in guinea pigs

作　　者：许险艳 杨维群 温扬敏 罗彩林 陈宜阳 

机构地区：[1]泉州医学高等专科学校

出　　处：《解剖学杂志》2015年第6期667-667,共4页Chinese Journal of Anatomy

基　　金：泉州医学高等专科学校校级重点科研项目(XJ1321)

摘　　要：目的:探讨豚鼠脑缺血再灌注损伤后螺旋神经节细胞凋亡诱导因子(AIF)表达的改变、细胞凋亡的情况以及两者之间的关系.方法:健康豚鼠40只随机分成正常组、假手术组和模型组,模型组分为缺血再灌注6、24、48h3组.用H-E染色观察脑缺血再灌注不同时间段耳蜗各部位的形态学改变,用免疫组织化学和免疫印迹检测螺旋神经节细胞AIF的表达部位和表达量,用原位末端凋亡检测法(TUNEL法)检测螺旋神经节细胞凋亡的情况.结果:正常组、假手术组螺旋神经节罕见凋亡细胞,AIF为阳性表达,表达部位在细胞质;模型组螺旋神经节在缺血再灌注每个时间段均有细胞凋亡,AIF为强阳性表达,表达部位在细胞核和细胞质,与正常组比较差异有统计学意义.结论:AIF参与了豚鼠脑缺血再灌注损伤后螺旋神经节细胞凋亡的发生.Objective:To study the relationship between apoptosis and expression of apoptosis inducing factor(AIF)following ischemia-reperfusion injury in cochlear of guinea pigs.Methods:Forty guinea pigs were selected and randomly divided into five groups:normal group,negative control group,ischemia-reperfusion 6,24 hours and 48 hours group.The morphology of cochlear was observed with hemotoxylineosin staining;the expression of AIF was studied by immunohistochemisty and Western blotting,and the cell apoptosis was studied by TUNEL.Results:In the ischemiareperfusion groups,labeling ratio of AIF expression was significantly higher compared with the normal and negative control groups.And there was almost no cell apoptosis in the normal and negative control groups.But there were more cells apoptosis in the ischemia-reperfusion groups.Conclusion:AIF may be an important part in the cell apoptosis of the cochlear of guinea pigs after ischemia-reperfusion injury.

关 键 词：耳蜗 缺血再灌注损伤 凋亡诱导因子 细胞凋亡 豚鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]