除草剂筛选小麦转基因植株的有效浓度研究  

Study on the Effective Concentration of Herbicide Screening for Transgenic Wheat

作　　者：李艺[1] 王悦[1] 王健[1] 赵翔宇[1] 王芳[1] 别晓敏[1] 

机构地区：[1]山东农业大学生命科学学院作物生物学国家重点实验室,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2016年第5期664-667,共4页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：泰安市大学生科技创新行动计划项目(2014D027)

摘　　要：利用筛选标记基因可方便快捷的区分转基因植株和非转基因植株。筛选标记基因通常用PCR、Southern杂交等分子手段检测。随着规模化转基因工作的开展,大量转基因植株需要及时准确的鉴定。叶片涂抹除草剂筛选转基因植株具有快速、高效的优势。本研究结果显示,除草剂草铵膦对小麦品种轮选987和科农199的有效筛选浓度分别为75 mg·L^(-1)和125 mg·L^(-1);对以轮选987为受体材料的转bar基因小麦植株的有效筛选浓度为100 mg·L^(-1),该浓度可用于针对此类转基因材料的大规模筛选。The selection marker gene was widely used to detect and identify transgenic plants in biotechnological breeding of crops. For detection of selection marker genes, the molecular techniques such as Polymerase-Chain-Reaction(PCR) and Southern blot were generally applied. However, with the rapid progress of genetic transformation of crop species, a large number of transgenic plants need to be identified timely and usually, bar was designed as the marker gene. The method painting herbicide(glufosinate-ammonium) on leaf has the advantage of quickness and effectiveness for verifying transformation. The results showed that in wheat varieties Lunxuan 987 and Kenong199, the effective selection concentrations of herbicide were 75 mg·L^(-1) and 125 mg·L^(-1), respectively. Further, the effective selection concentrations of herbicide were 100 mg·L^(-1) for detecting the activity of bar protein when Lunxuan 987 was transformed as the donor plant,suggesting this concentration of glufosinate-ammonium might be suitable for large scale detection.

关 键 词：小麦 转基因 除草剂 BAR基因 有效浓度 

分 类 号：S512.1[农业科学—作物学] S482.4