多探头光纤倏逝波生物传感器及其性能研究  

Multi-Probe Fiber-Optic Evanescent Wave Biosensorand Its Characterization

作　　者：黄惠杰[1] 翟俊辉[2] 赵永凯[1] 杨瑞馥[2] 任冰强[1] 程兆谷[1] 杜龙龙[1] 路敦武[1] 

机构地区：[1]中国科学院上海光学精密机械研究所,上海201800 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国激光》2004年第6期718-722,共5页Chinese Journal of Lasers

摘　　要：利用光纤倏逝波原理 ,以输出波长为 6 35nm的半导体激光器为光源 ,研制成功一台具有 5个探头的光纤生物传感器。该传感器 5个光纤探头对纯净Cy5荧光染料溶液的检测灵敏度均达 0 0 1nmol/L ,在同一浓度下信噪比的相对标准偏差小于 10 % ,5个探头的信噪比曲线几乎重合 ,且与商品化生物芯片扫描仪同时检测得到的结果一致 ;检测到了抗原抗体特异性反应的动态过程。本传感器具有较高的检测灵敏度、良好的响应一致性和生物特异性 ,可用于多重生物物质的检测。A multi-probe fiber-optic evanescent wave biosensor has been developed based on the principle of evanescent wave while light propagates along optical fiber. A laser diode at 635 nm wavelength is used as the light source. The biosensor can interrogate five individual fibers at one time. Thus assays for five separate analytes can be conducted on the same sample. For all the five fibers, sensitivity limit of 0.01 nmol/L is obtained from the detection of Cy5 serial solutions with various concentrations. The response curves of five fibers have good consistency, the relative standard deviation is less than 10%. All the results are comparable with those that are obtained by a commercial biochip scanner. Real-time fluorescence of human IgG binding to goat anti-body IgG antibody immobilized on fiber core has been successfully detected.

关 键 词：传感器技术 光学计量 生物传感器 倏逝波 蛋白质 实时荧光检测 

分 类 号：TP212.14[自动化与计算机技术—检测技术与自动化装置]