ehCGβ肿瘤基因疫苗诱生的效应脾细胞过继免疫抗肿瘤作用的研究  

Anti-Tumor Effects of Adoptively Transferred Splenocytes Induced by ehCGβ Gene Immunization

作　　者：关庆东[1,2] 王立新[1,2] 张进平[1,2] 徐薇[1,2] 储以微[1,2] 王缨[1,2] 熊思东[1,2] 

机构地区：[1]复旦大学上海医学院免疫学系教育部分子医学重点实验室 [2]上海基因免疫与疫苗研究中心,上海200032

出　　处：《中国肿瘤生物治疗杂志》2004年第2期79-83,共5页Chinese Journal of Cancer Biotherapy

基　　金：上海市科技攻关重点项目(03dz19229);国家杰出青年科学基金研究计划(39925031)

摘　　要：目的:探讨ehCGβ肿瘤基因疫苗诱生的效应脾细胞过继免疫在抗肿瘤中的作用。方法:通过转染建立稳定表达ehCGβ和HBV—preS2/S的细胞株Sp2/0-ehCGβ和Spz/0-preS2/S。以TR421-hCGβ质粒实施基因免疫,免疫后检测小鼠脾细胞,特异性细胞毒活性;同期将脾细胞过继免疫给正常小鼠,以过继TR421-hCGβ质粒免疫小鼠脾细胞为实验组、过继TR421质粒免疫小鼠脾细胞为对照组,检测脾细胞杀伤效应。结果:效应脾细胞对sp2/0-ehCGβ的杀伤率明显高于Sp2/0—preS2/S(P<0.05)。Sp2/0-ehCGβ在实验组小鼠仅2只形成实体瘤,TR421-hcGβ质粒基因免疫抗肿瘤任用有肿瘤特异性,两组有显著性差异(P<0.05),而在对照组小鼠均形成实体瘤。Sp2/0-preS2/S在所有的小鼠均形成了实体瘤,其瘤重无显著性差异。结论:ehCGβ肿瘤基因疫苗诱生的效应细胞可特异性杀伤肿瘤,过继免疫效应细胞能使正常小鼠获得明显的特异性抗肿瘤能力。Objective: To investigate the anti-tumor effects of adoptively transferred splenocytes induced by gene immunization with ehCGβ tumor vaccine. Methods: Sp2/0 cells were transfected with the plasmids containing ehCGβ or HBV-preS2/S and the postive clones were screened by G418. BALB/c mice were immunized with plasmid TR421-hCGβ or mock DNA three times. Two weeks after immunization, spleen cells from the immunized mice were harvested, and then analyze the CTL activity against Sp2/0-ehCGβ cells. The splenocytes derived from the immunized mice were adoptively transferred to the normal mice, which were subsequently given injections i. p. of Sp2/0-ehCGβ and β Sp2/0-preS2/S respectively. Results: Splenocytes derived from the mice immunized with TR421-hCGp exhibited a strong specfic cytotoxic activity against Sp2/0-ehCGβ cells, The average tumor weight between test and control group is statistically significant ( P < 0. 05 ) . Only two mice out of six had tumors in Sp2/0-ehCGβadoptively transferred group, while all mice had tumors in control group. However, all mice challenged with Sp2/0-preS2/S formed tumors, and the average tumor weight was not obviously varied between the two groups. Conclusion: ehCGβ gene vaccine could induce specific cytotoxic splenocytes a-gainst ehCGβ and adoptive transfer of the splenocytes showed the anti-tumor activity.

关 键 词：异位人绒毛膜促性腺激素 效应脾细胞 肿瘤 基因疫苗 过继免疫 

分 类 号：R730.59[医药卫生—肿瘤]