中药复方髓伤平调节大鼠脑内自由基作用的研究  

Regulation of Oxygen Free Radical in Rat Brain with Chinese Herbs, Suishangping

作　　者：孙丛晓[1] 黄长胜[1] 韩凤岳[1] 刘娜[2] 陈亚亮[2] 杨琳[2] 

机构地区：[1]中国中医研究院骨伤科研究所基础实验室 [2]首都医科大学解剖学教研室

出　　处：《首都医科大学学报》2004年第2期147-149,共3页Journal of Capital Medical University

基　　金：北京市科学技术委员会基金 ( 95 40 2 2 0 0 0 )资助项目

摘　　要：为了探讨中药复方髓伤平 (SSP)对全脑缺血再灌流大鼠模型脑保护作用的机制 ,阻断 1 8只SD大鼠双侧颈总动脉血流 3 0min后 ,解除阻断再灌流 2h ,造成脑缺血再灌流损伤模型。造模大鼠随机分为 3组 :缺血再灌流组(BG) ,SSP治疗组 (SG) ,尼莫地平对照组 (NG) ;另设不造模的正常对照组 (NCG) 6只。分别检测 4组大鼠脑组织内丙二醛 (MDA)质量摩尔浓度及超氧化物歧化酶 (SOD)活性。发现 :BG组大鼠脑内MDA质量摩尔浓度明显升高 (P <0 .0 5 ) ,SOD活性则明显下调 (P <0 .0 5 )。SSP能使升高的MDA适度降低 ,并且上调SOD活性。提示 :在大鼠脑缺血再灌流条件下 ,SSP可以清除脑内过多的氧自由基 ,对脑起保护作用。The objective was to observe the protective effect of Chinese herbal compound, Suishangping (SSP) on ischemia reperfusion injury of the brain. Altogether 24 Sprague-Daleg rats were randomly divided into four groups: normal control group (NCG), ischemic injury group (BG), SSP group (SG) and nimodipine group (NG). It was followed by reperfusion of blood for 2 h to blockade blood flow in the common carotid artery with silk ligature for 30 mins in BG, SG, and NG. SOD activity and MDA content were detected in various groups. It has been demonstrated that MDA value in rats-SG was lower than that in rats-BG (P<0.05), and SOD activity in rats-SG was higher than that of BG ( P< 0.05). It shows that SSP can reduce the contents of MDA, and stimulate SOD activity, so that it maybe indirectly resist the damage of oxygen-derived free radicals.

关 键 词：中药复方 髓伤平 自由基 脑保护作用 丙二醛 超氧化物歧化酶 

分 类 号：R285.6[医药卫生—中药学]