胰腺炎相关蛋白与结直肠癌DNA倍体性的关系  

Relationship Between Expression of Pancreatitis Associated Protein and Cell Ploidy in Colorectal Cancer Tissues

作　　者：曹广[1] 李非[2] 孙家邦[2] 

机构地区：[1]首都医科大学附属北京安贞医院普外科 [2]首都医科大学宣武医院普外科

出　　处：《首都医科大学学报》2004年第2期243-245,共3页Journal of Capital Medical University

摘　　要：为探讨结直肠癌组织中胰腺炎相关蛋白 (PAP)mRNA表达与肿瘤DNA倍体性的关系 ,收集结直肠癌外科手术标本 2 7例 (病理均证实为腺癌 ) ,同时取距肿瘤边缘 3cm和 8cm的黏膜组织作为对照。采用逆转录聚合酶链技术 ,测定样本中有无胰腺炎相关蛋白的表达 ,并用流式细胞仪对各样本行细胞DNA倍体性分析。发现 :PAPmRNA在结直肠癌中的表达阳性率为 2 9.6% ( 8/2 7)。在 8例PAPmRNA阳性表达的肿瘤组织样本中 ,S期细胞比率 (SPF)为 1 8.91± 8.43 ,并有 7例为非整倍体肿瘤 ;而另外 1 9例PAPmRNA阴性肿瘤组织的SPF为 1 2 .1 1± 7.0 2 ,有 4例为非整倍体肿瘤。在B、C 2个对照组的黏膜组织中未能检测出PAPmRNA阳性表达及非整倍体组织。经统计学处理PAPmRNA阳性样本的非整倍体例数和SPF ,与阴性样本相比差异有显著性 (P <0 .0 5 ) ;而肿瘤细胞的分化程度和扩散范围与PAPmRNA的阳性表达无明显相关。提示 :PAPmRNA阳性肿瘤样本有较高增生活性 ,PAPmRNA的表达与肿瘤分化、浸润深度及淋巴结转移情况无关。The aim was to investigate the reationship between expression of pancreatitis-associated protein and cell DNA ploidy in colorectal cancer tissues. All fresh samples were obtained from 27 colorectal adenocarcinoma patiens. The expression of PAP mRNA was examined by reverse transcription-polymerase chain reaction(RT-PCR), while the s-phase fraction(SPF) and ploidy of DNA were examined by flow cytometry(FCM). In this assay, PAP mRNA was detected in 8(29.6%) colorectal cancer tissues, for these samples SPF was 18.91±8.43, and had 7 aneuploid cancer tissues. However only 4 aneuploid cases were found in 19 patients who had negative expression of PAP mRNA in cancer tissues and the value of SPF was 12.11±7.02. On the other hand, expression of PAP mRNA and aneuploid tissues were not detected in noncancerous portions. For DNA ploidy and SPF, there was significant difference between PAP mRNA positive and negative cancer tissues. So these PAP mRNA positive cancer tissues have higher proliferative active. But there is no relationship between PAP mRNA expression and clinicopathological factors.

关 键 词：胰腺炎相关蛋白 结直肠癌 流式细胞学 增生活性 

分 类 号：R735.35[医药卫生—肿瘤]