建立一种脑梗死血瘀证大鼠模型的实验研究  

Experimental Study on Establishment a Rat Model of Cerebral Infarction in Blood Stasis Syndrome

作　　者：朱惠斌[1] 陈懿[2] 葛金文[2] 

机构地区：[1]湖南中医学院第一附属医院,湖南长沙410007 [2]湖南中医学院,湖南长沙410007

出　　处：《湖南中医药导报》2004年第6期77-79,83,共4页Hunan Guiding Journal of Traditional Chinese Medicine and Pharmacology

基　　金：湖南省中青年基金资助课题 (0 0JZY2 14 5 )

摘　　要：目的 :建立一种因血管内皮细胞损伤而诱导的大鼠局灶性脑梗死血瘀证模型。方法 :取SD大鼠 ,分离左侧颈总动脉、颈外动脉和颈内动脉 ,结扎左颈外动脉远心端 ,夹闭左翼腭动脉和颈总动脉 ,经颈外动脉逆行插管并缓慢注入月桂酸钠至颈内动脉系统 ,造成大脑中动脉血管内皮细胞受损。结果 :从模型动物的行为表现、梗死灶形态学和病理学及血液流变学等方面考察 ,大鼠可产生一定程度的脑梗死和血瘀证表现 ,造模成功率为 90 .9%。结论 :该模型的稳定性和重复性较好 ,能在大脑中动脉的供血区产生恒定的梗死灶 ,适合进行中医药防治脑梗死血瘀证研究。Objective: To establish a rat model of focal cerebral infarction in blood stasis syndrome caused by endothelial cell injury. Methods: Splitting left-sidedness common carotid artery, external carotid artery and internal carotid artery, deligating left external carotid artery, closuring palatine arteries and common carotid artery by clip, injecting clinibolin in internal carotid artery from external carotid artery by retrocatheteris, the endothelial cell in middle cerebral artery was injured. Results: Studying from the performance of the model animal, the morphology, pathology and hemorheology about focus infarction, the rat has some manifestation of cerebral infarction with blood stasis syndrome. The success rate of the model was 90.9%. Conclusion: The stability and repeatability of the model were good for study on Chinese Medicine for the prevention and treatment of cerebral infarction with constant focal infarct in middle cerebral artery.

关 键 词：脑梗死 血瘀证 动物模型 

分 类 号：R228[医药卫生—中医基础理论]