Purification and Characterization of Cytotoxins from Agkistrodon acutus Venom and Their Anticancer Activity  

中国皖南尖吻蝮蛇毒中细胞毒素的分离纯化和抗癌活性研究(英文)

作　　者：章良[1] 李虹[1] 吴梧桐[1] 

机构地区：[1]中国药科大学生物制药学院,南京210009

出　　处：《Journal of Chinese Pharmaceutical Sciences》2004年第2期97-102,共6页中国药学（英文版）

基　　金：NationalNaturalScienceFundationofChina( 3 0 17110 3 )

摘　　要：Aim To investigate the anticancer activity of two new cytotoxins from thevenom of Agkistrodon acutus. Methods The venom was isolated by FPLC column chromatography consistingof DEAE Sepharose FF and Source 30S. The cytotoxic activity on tumor cells was detected by MITmethod. Purity and molecular weight were determined by SDS-PAGE (silver staining). Their stabilitiesto temperature and pH were also detected. Results Two pure cytotoxins named ACTX-6 and ACTX-8 wereobtained. Their molecular weights are 98 kDa and 27 kDa, respectively. ACTX-6 consists of twosubunits bonded together by disulfide bonds. Conclusion ACTX-6 and ATCX-8 have highest inhibitoryactivity on lung cancer cell A549. ACTX-6 is stable to heat while ACTX-8 not. ACTX-6 is stablebetween pH 7-9 and ACTX-8 between pH 6 - 9.目的　寻找皖南尖吻蝮蛇毒中的抗癌组分并初步研究其性质。方法　以DEAE SepharoseFF ,SOURCE 30S柱层析纯化粗毒 ;以MTT法检测细胞毒活性 ;以SDS PAGE(银染 )测定纯度 ;以还原性及非还原SDS PAGE测定分子量 ;又测定了它们的热稳定性和pH稳定性。结果　得到两个电泳纯的蛇毒类抗癌组分 ,分别命名为ACTX 6和ACTX 8,分子量为 98kDa和 2 7kDa ,都不是糖蛋白 ,其中ACTX 6由两个分子量相近的亚单位以二硫键连接而成 ;它们对肺癌细胞A5 4 9最敏感 ;ACTX 6对热稳定而ACTX 8对热不稳定 ;ACTX 6的稳定pH范围为 7- 9,而ACTX 8在 6 -

关 键 词：agkistrodon acutus snake venom anticancer activity CYTOTOXIN columnchromatography 

分 类 号：R99[医药卫生—毒理学]