Isolation and Purification of Gonyautoxins from Two Strain of Alexandrium minutum Halim  

从两株微小亚历山大藻中分离纯化膝沟藻毒素(英文)

作　　者：缪宇平[1] 陈逸民[2] 周宏农[2] 闻韧[3] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090 [2]台湾大学理学院,台北10617 [3]复旦大学药学院,上海200032

出　　处：《Journal of Chinese Pharmaceutical Sciences》2004年第2期103-105,共3页中国药学（英文版）

摘　　要：Aim To study isolation and purification of Gonyautoxins from two differentstrains of Alexandrium minutum Halim. Methods Ethanol extracts of the two culture strains ofAlexandrium minutum Halim were fractionated by gel filtration and ion exchange chromatography toobtain gonyautoxins. The toxin profiles were tested by HPLC method. Results Both strains containgonyautoxin-1, gonyautoxin-2, gonyautoxin-3 and gonyautoxin-4. The total gonyautoxin content ofAmtk4 strain is almost 10 times higher than that of Amtk2 strain. Conclusion Alexandrium minutumHalim Amtk4 strain is suitable for the preparation of Gonyautoxins.目的　从两株微小亚历山大藻 (AlexandriumminutumHalim)中分离纯化膝沟藻毒素 (Gonyautoxins)。方法　用胶滤层析、离子交换层析等方法从人工培养的两株微小亚历山大藻酸酒精萃取物中分离纯化膝沟藻毒素 ,再用HPLC法对毒素进行定性定量测定。结果　从两株藻株培养液中均分离得到膝沟藻毒素GTX 4 ,GTX 1,GTX 3,GTX 2 ,其中Amtk4藻株的毒素含量为Amtk2的近 10倍。

关 键 词：alexandrium minutum halim gonyautoxins gel filtration chromatography ionexchange chromatography 

分 类 号：R914[医药卫生—药物化学]