Qualitative and Quantitative Analysis of Rhizoma of Polygonum cuspidatum  

中药虎杖商品药材的定性鉴别与定量分析(英文)

作　　者：张勉[1] 王磊[1] 黄澜[1] 张紫佳[1] 王峥涛[1] 

机构地区：[1]中国药科大学生药学研究室,南京210038

出　　处：《Journal of Chinese Pharmaceutical Sciences》2004年第2期106-111,共6页中国药学（英文版）

基　　金：TheNinth Five yearNationalKeyScientificandTech nicalProgramofChinaNo.99 92 9 0 1 3 1

摘　　要：Aim To establish reliable methods for evaluating the quality of rhizoma of Polygonum cuspidatum( Huzhang in Chinese). Methods TLC and HPLC were employed for the chemical identification and content determination,respectively. Results A qualitative TLC method and a quantitative HPLC method with piceid as the reference substance were established, respectively. With piceid as the reference substance and ethyl acetate-methanol-formic acid-water ( 19:3:0.5:1) as the mobile phase, a TLC method for the identification of Huzhang from the commonly used crude drugs of the same family was also set up. Conclusion The established TLC method can reasonably appraise the quality of the drug and easily distinguish Huzhang from the other commonly used crude drugs of the same family. The HPLC method for determining piceid is simple, reproducible, accurate, and feasible.目的　建立较为合理的中药虎杖的质量评价标准。方法　化学定性鉴别采用TLC法 ,含量测定采用HPLC法。结果　以白藜芦醇苷和大黄素为对照品 ,以不同的展开系统展开 ,建立了虎杖的TLC定性鉴别方法 ;以白藜芦醇苷为对照品建立了HPLC含量测定方法。结论　建立的TLC方法对虎杖药材的鉴别具有较好的专属性 ,能够区别虎杖与同科属常用药材(2 0 0 0年版中国药典收载的同科根与根茎类药材 )。本文建立的测定白藜芦醇苷含量的HPLC法简单、准确 。

关 键 词：TLC HPLC PICEID EMODIN rhizoma of Polygonum cuspidatum 

分 类 号：R282.5[医药卫生—中药学]