胃癌组织iNOS、COX-2和VEGF的表达及其与血管生成关系  

Expression of inducible nitric oxide synthase, cyclooxygenase-2 and Vascular endothelial growth factor in human gastric cancer and their relationship to angiogenesis

作　　者：盛霞[1] 吴继锋[1] 秦蓉[1] 王道斌[1] 张红[1] 

机构地区：[1]安徽医科大学病理教研室,合肥230032

出　　处：《安徽医科大学学报》2004年第3期208-212,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省卫生厅 (编号 :2 0 0 2A0 2 8);省教育厅 (编号 :2 0 0 3kj183 )科研基金资助

摘　　要：目的　探讨iNOS、COX 2、VEGF在人胃癌组织中的表达及其与胃癌血管生成及临床病理因素之间的关系。方法　应用免疫组化S P法检测 6 7例胃癌组织中iNOS、COX 2、VEGF的表达 ,根据第八因子相关抗原 (FⅧRAg)阳性的血管内皮细胞计数肿瘤组织微血管密度 (MVD)。结果　①iNOS、VEGF阳性表达主要定位于癌细胞胞质 ;COX 2的阳性表达定位于癌细胞的胞质 ,部分胞膜见阳性表达 ;胃癌组织iNOS、COX 2和VEGF高表达率分别为 6 8 7% ,76 1%和 76 1% ;②iNOS、COX 2和VEGF的高表达与胃癌组织的分化程度成正相关 ;③癌组织MVD均数为 30 84± 9 13个 /2 0 0倍视野 ,iNOS、COX 2及VEGF高表达组MVD显著高于低表达、无表达组 ,差异具有显著性意义 (P值均 <0 0 5 )。④iNOS、COX 2和VEGF高表达与胃癌浸润深度呈显著正相关 (P <0 0 0 1) ,iNOS、VEGF的高表达还与五年生存率呈负相关 (P <0 0 1)。⑤iNOS、COX 2的表达均与VEGF的表达呈正相关 (r分别 =0 5 2 7,0 4 2 5 ;P值分别 =0 0 15 ,<0 0 0 1)。结论　胃癌组织iNOS、COX 2的表达与肿瘤血管生成密切相关 ,二者可能与VEGF协同促进肿瘤血管生成 ,有望成为判断肿瘤恶性潜能的重要生物学指标。Object To examine the expression of inducible nitric oxide (iNOS), cyclooxygenase-2 (COX-2),Vascular endothelial growth factor (VEGF) in human gastric cancer (GC) and their relationship to angiogenesis and clinical pathology.Methods Tissue sections from 67 GC patients were examined using immunohistochemistry technique for the expression of iNOS,COX-2 and VEGF;Microvascular density (MVD) was measured by endothelial cells immunostained by anti-FⅧAg antibodies.Results iNOS,VEGF expression was located in the cytoplasm of GC cells while COX-2 protein was located in the cytoplasm and membrance of GC cells.The positive rate of iNOS,COX-2 and VEGF in GC was 68.7%,76.1% and 76.1% respectively.Cancer MVD was significantly correlated with the expression of iNOS,COX-2 and VEGF.Moreover, the expression of iNOS,COX-2,VEGF was significantly correlated with the depth of invasion (P<0.001).There was a significant positive correlation between the expression of iNOS,COX-2 and VEGF.Conclusion iNOS,COX-2 may promote angiogenesis through the way of VEGF,they may be two important biological markers in evaluating the malignant degree and angiogenesis in GC.

关 键 词：胃肿瘤 新生血管化 病理学 一氧化氮合酶 环氧合酶-2 血管内皮生长因子 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤] R364.3[医药卫生—临床医学]