大鼠Hp相关性慢性胃炎模型的制做  

Study of Rat Model of Hp-associated Chronic Gastritis

作　　者：张琳[1] 姚冬梅[2] 姚希贤[2] 王娜[2] 姚金峰[2] 赵丽梅[2] 

机构地区：[1]北京中医医院消化科,北京100010 [2]河北医科大学第二医院消化研究所

出　　处：《中国比较医学杂志》2004年第3期161-165,共5页Chinese Journal of Comparative Medicine

摘　　要：目的　建立较理想的幽门螺杆菌 (Hp)相关性慢性胃炎大鼠模型。方法　二级雄性Wister大鼠 4 3只 ,随机分为制模组 [H .pylori及 1 甲基 3 硝基 1亚硝基胍 (N methyl N’ nitro N nitrosoguanidine ,MNNG)组 ]1 3只、对照Ⅰ组 (正常对照组 ) 1 0只 ,对照Ⅱ组 (H .pylori感染组 ) 1 0只 ,对照Ⅲ组 (MNNG组 ) 1 0只。对模型组动物 ,采用H .pylori及MNNG的综合方法制模 ,共 1 2周。采用胃黏膜涂片革兰染色和快速尿素酶试验检测H .pylori定植情况。并对胃窦黏膜组织学各项指标进行评定。结果　 (1 )H .pylori感染情况 :正常对照组和MNNG组 :其胃窦黏膜无H .pylori感染 ;H .pylori感染组和制模组分别处死 9只、1 0只大鼠 ,其中 8/9、7/1 0胃窦黏膜有H .pylori定植。 (2 )胃窦黏膜病理组织学变化 :制模组胃窦黏膜变薄 ,固有层腺体中度减少 ,未见腺体化生及不典型增生 ,伴有中度慢性活动性炎症。MNNG组胃窦黏膜变薄 ,固有层腺体中度减少 ,亦无腺体化生及不典型增生。伴轻度慢性炎症 ,但无明显急性活动性炎症。H .pylori感染组仅有中度慢性活动性炎症 ,胃窦黏膜厚度正常。正常对照组胃黏膜基本正常。制模组慢性活动性炎症与H .pylori感染组比较 ,差异无显著意义 ,P >0 0 5。萎缩性病变 [病理积分 (2 2±0 4Objective To set up a better rat model of %H.pylori% infected chronic gastritis.Methods 43 grade Ⅱ male Wister rats were divided randomly into 4 groups, i.e. model group[%H.pylori%, N methyl N’ nitro N nitrosoguanidine(MNNG) group, 13 rats], control group Ⅰ(normal control group, 10 rats), control group Ⅱ(%H.pylori% group, 10 rats), control group Ⅲ(MNNG group,10 rats). The combined application of %H.pylori% with MNNG was done in model making for 12 weeks. Both Gram staining of gastric antrum mucosal biopsy and rapid urease testing were made to detect the colonization of %H.pylori%, and to assess the histological indexes for gastric antrum mucosa. Results (1) %H.pylori% infection: For normal control group and MNNG group, no colonization of %H.pylori% was found in rats. For %H.pylori% group, eight of the nine killed rats were found to be colonized in gastric mucosa by %H.pylori%, while for model group, seven of the ten killed rats were found to be in the same location.(2)Histopathologic changes of gastric antrum mucosa:For the rats from model group, gastric antrum mucosa became thinner, glands in lamina propria decreased moderately, and neither glandular metaplasia nor atypical hyperplasia was found, but moderate chronic active inflammation noted. For the rats from MNNG group, gastric antrum mucosa became thinner, glands in lamina propria decreased moderately, neither glandular metaplasia nor atypical hyperplasia was found, however, mild chronic inflammation was found while no distinguishable acute active inflammation noted. For the rats from %H.pylori% group, only moderate chronic active inflammation was found, the thickness of gastric antrum mucosa kept normal. For the rats from normal control group, gastric antrum mucosa was basically normal.There was no remarkable difference in chronic active inflammation between model group and %H.pylori% group, %P%>0 05, and also no remarkable difference in atrophic changes between model group (pathologic score 2.2±0 42 points) and MNNG group (pathologic

关 键 词：大鼠 HP 慢性胃炎 动物模型 螺杆菌 

分 类 号：R573.3[医药卫生—消化系统]