高温低盐压力对缢蛏血淋巴碱性强白影响机理的研究  

Influence mechanisms of high temperature and low salinity on hemolymph basic proteins of Sinonovacula constricta

作　　者：张鹭[1] 陈寅山[2] 彭宣宪[3] 

机构地区：[1]齐齐哈尔大学生命科学院,黑龙江齐齐哈尔161005 [2]福建师范大学生物工程学院,福建福州350007 [3]厦门大学生命科学院,福建厦门361005

出　　处：《中国海洋药物》2004年第3期23-27,共5页Chinese Journal of Marine Drugs

基　　金：福建省教育厅资助项目(A类)(项目编号:JA02179)

摘　　要：以缢蛏Sinonvacula constricta (Lamarck)为研究对象,用双相电泳及图像分析等蛋白质组学技术探求其在高温、低盐2种生态压力下血淋巴内40KDa以下小分子碱性蛋白质的差异表达,以寻找缢蛏血淋巴的抗性机制,明确血淋巴在机体防御体系中的作用。 在环境压力下,缢蛏血淋巴内有多种蛋白发生变化,MALDI-TOF-MS检测鉴定出磷脂酶A2、表皮分泌蛋白xp2前体、脑衍生神经营养因子、肿瘤坏死因子受体、脯氨酸顺反同工酶等7种蛋白或多肽。涉及多种与炎症反应、基质转运、神经营养、基因表达调控、蛋白质合成等有关的功能。说明其血淋巴在缢蛏的防御体系中具有关键性作用。Sinonovacula constricta(Lamarck), a kind of mollusks, is an important species of marine aquaculture. We choose it as our experiment sample and its basic protein expression profiles of hemolymph after short-term treatment ur(?)der high temperature and low salinity were detected, seven kinds of proteins or polypeptides were identified: Cysteine-rich protein 2 CRP2, Phospholipase A2, Pepidyl-prolyl cis- trans isomerase A, epidermal secretory protein xp2 precursor, brain-derived neurotrophic factor, tumor necrosis factor receptor, highmobility groupprotein HMGI-C. They have multiple functions, such as nutrition, gene expression regulation, protein synthesis, substances transport, antitoxin and anti-interference. The results suggested that the hemolymph played a key action in defense System of Sinonovacula constricta .

关 键 词：双相电泳 图像分析 缢蛏 血淋巴碱性蛋白 高温低盐 

分 类 号：R959.251[医药卫生—药学]