人颌骨骨膜成骨细胞复合异体部分脱钙骨的成骨实验研究  

TISSUE ENGINEERED BONE WITH HUMAN OSTEOBLASTS AND ALLOPLASTIC PARTIALLY DECALCIFIED BONE

作　　者：陈伟良[1] 藕小平[1] 王建广[1] 杨朝晖[1] 李海刚[2] 

机构地区：[1]中山大学附属第二医院口腔颌面外科,广州510120 [2]中山大学附属第二医院病理科,广州510120

出　　处：《口腔颌面外科杂志》2004年第2期112-114,共3页Journal of Oral and Maxillofacial Surgery

摘　　要：目的　研究人颌骨骨膜来源的成骨细胞接种于异体部分脱钙骨内组织工程化成骨的可行性。方法　从人颌骨骨膜分离培养成骨细胞 ,观察其体外生长情况 ,将扩增的人成骨细胞接种到异体部分脱钙松质骨支架内 ,体外和裸鼠体内培养 ,观察成骨细胞在支架孔隙内粘附、生长和成骨情况。结果　人颌骨骨膜来源的成骨细胞体外生长良好 ,细胞在异体部分脱钙松质骨支架内能正常的粘附、生长 ,裸鼠皮下培养 2月后 ,支架材料内异位形成岛状的新生骨组织。Objective To investigate the feasibility of tissue engineered bone by human mandbular periosteum osteoblasts seeded into alloplastic partially decalcified bone. Methods Human osteoblasts isolated from mandibular periosteum were cultured in vitro and observed under inverse microscope. Human osteoblasts were seeded into alloplastic partially decalcified spong bone. The composite was cultured in vitro for 4 days then implanted into subcutaneous pockets on the dorsum of athymic mice for 2 months, the cells'growth, adhension and bone formation were observed. Results The osteoblasts proliferated well after they were cultured in vitro. The cells adhered well to the scaffold materials. 2 months later, new bone was observed. Conclusion It is possible to fabricate bone by seeding human osteoblasts into alloplastic partially decalcified bone.

关 键 词：颌骨 骨膜成骨细胞 复合异体 脱钙骨 成骨实验 异体脱钙骨 

分 类 号：R782.2[医药卫生—口腔医学]