压力对髁突软骨细胞超微结构及可聚蛋白多糖分泌的影响  

Effects of Mechanical Pressure on the Ultrastructure and the Aggrecan Synthesis in Mandibular Condylar Chondrocytes

作　　者：张旻 王美青[1] 王景杰[1] 

机构地区：[1]第四军医大学口腔医学院解剖生理教研室,陕西西安710032

出　　处：《医用生物力学》2004年第2期69-73,共5页Journal of Medical Biomechanics

基　　金：国家自然科学基金资助 (No 3 0 0 0 0 0 3 5 )

摘　　要：目的　探讨髁突软骨细胞超微结构及软骨可聚蛋白多糖 (aggrecan)分泌随压力的变化过程。 方法　体外培养髁突软骨细胞 ,采用可控液压细胞加载装置 90kPa压强下分别加载 6 0min、36 0min ,透射电镜观察细胞的超微结构 ,原位杂交方法观察髁突软骨细胞 (MCC)中软骨可聚蛋白多糖mRNA的表达。结果　对照组细胞在透射电镜下生长良好 ,未见到细胞凋亡现象 ;90kPa加压 6 0min后细胞生长状态亦良好 ,多见到明显伸长盘绕的微绒毛 ;90kPa加压 36 0min后细胞部分出现明显的细胞凋亡指征 ;软骨可聚蛋白多糖mRNA在受压 6 0min后表达明显增强 ,加压时间延长到 36 0min后表达弱于 6 0min组但仍高于对照。结论　适宜的压力可使MCC结构及功能都发生一定的变化 ,aggrecan代谢水平升高。Objective To investigate the change of the ultrastructure and the variation of synthesis in aggrecan,the main proteoglycan component of the cartilage matrix of mandibular condylar chondrocytes(MCC), under the mechanical pressure. Methods In vitro cultured MCC from two-week-old New Zealand rabbits were incubated under continuous pressure of 90 kPa for 60min,360min by hydraulic pressure controlled cellular strain unit.The ultrastructure of MCC was observed under transmission electron microscope(TEM).In situ hybridization method was used to test aggrecan mRNA expression in MCC. Results The appearance of MCC on TEM showed that the cells of the control developed in the normal way.After been pressed under 90kPa for 60min,the cells showed elongated and voluminous cellular processes with increasing aggrecan mRNA.When the pressure lasted to 360 min,some of the cells showed distinct sign of apotosis and the aggrecan mRNA decreased but still higher than that of the control. Conclusion Continuous pressure could cause changes on the ultrastructure and function of MCC.Pressure may be used to up-regulate the synthesis of aggrecan.

关 键 词：髁突软骨细胞 压力 软骨可聚蛋白多糖 透射电镜 

分 类 号：R780.2[医药卫生—口腔医学]