结直肠癌雌激素受体及孕激素受体的定量研究  

Expression of Estrogen Receptor and Progesterone Receptor in Colorectal Cancer: A Quantitative Study

作　　者：周志伟[1] 万德森[1] 王国强[1] 潘志忠[1] 卢汉平[2] 高锦辉[2] 丁培荣[1] 

机构地区：[1]中山大学肿瘤防治中心腹科,广东广州510060 [2]中山大学中山医学院实验核医学教研室,广东广州510089

出　　处：《癌症》2004年第7期851-854,共4页Chinese Journal of Cancer

摘　　要：背景与目的许多研究表明结直肠癌与雌激素相关,结直肠癌组织中表达雌激素受体(estrogenreceptor,ER)、孕激素受体(progesteronereceptor,PR),但多采用免疫组化方法检测,且结果不一,而ER.PR的定量研究不多。本研究拟定量测定结直肠癌组织及正常组织的ER,PR表达水平,并探讨它们之间的关系及与结直肠癌患者临床病理参数的关系。方法应用受体放射配基结合分析法(radioligandbindingassay,RBA)定量测定45例结直肠癌组织及结直肠正常组织的胞浆及胞核ER、PR的水平。结果45例标本结直肠正常组织及癌组织都表达ER、PR,胞浆ER表达癌组织高于正常组织,分别为7.96±3.69fmol/mgprotein和4.34±2.84fmol/mgprotein,(P<0.01);胞浆PR表达癌组织高于正常组织,分别为3.89±2.64fmol/mgprotein和2.50±1.73fmol/mgprotein,(P<0.01);胞核ER表达癌组织高于正常组织,分别为18.42±8.30fmol/mgprotein和11.24±5.44fmol/mgprotein,(P<0.01);胞核PR表达癌组织高于正常组织,分别为9.36±5.90fmol/mgprotein和7.84±7.41fmol/mgprotein,(P<0.05)。癌组织胞浆及胞核的ER表达与PR表达呈正相关,(P<0.01);正常结直肠组织胞浆ER表达与PR表达呈正相关,(P<0.01),而胞核内两者不相关,(P>0.05)。结直肠癌组织ER表达与患者年龄相关,大于45?BACKGROUND & OBJECTIVE: It was indicated that there was relationship between estrogen and colorectal cancer. Estrogen receptor (ER) and progesterone receptor (PR) were expressed in colorectal cancer tissue. They were measured using immunohistochemistry with various results,but there was little quantitative study. This study was designed to quantitatively measure the expression of ER and PR in tumor tissues and normal mucosas and analyze its relationship with clinicopathological parameters in colorectal cancer. METHODS: ER and PR expression in cytoplasm and nucleus of tumor tissues and normal mucosas from 45 colorectal cancer patients were quantitatively measured using radioligand binding assay (RBA). RESULTS: ER and PR were expressed in all tumor tissues and normal mucosas. In cytoplasm, the ER expression level of tumor tissues was higher than that of normal mucosas; they were 7.96±3.69 fmol/mg protein and 4.34±2.84 fmol/mg protein (P< 0.01) respectively. And that was the same for PR expression; they were 3.89±2.64 fmol/mg protein and 2.50±1.73 fmol/mg protein(P< 0.01)respectively. In nucleus, the ER expression level of tumor tissues was higher than that of normal mucosas; they were 18.42±8.30 fmol/mg protein and 11.24±5.44 fmol/mg protein (P< 0.01)respectively. And that was the same for PR expression, they were 9.36±5.90 fmol/mg protein and 7.84±7.41 fmol/mg protein (P< 0.05) respectively. There was positive correlation between ER and PR expression in cytoplasm and nucleus of tumor tissues (P< 0.01) respectively,so was in cytoplasm in normal mucosas (P< 0.01)respectively, but not in nucleus (P >0.05). There was correlation between ER expression in tumor tissues and patients age,and the expression in these people over 45 years old was higher than those less 45 years old (P< 0.05), but no correlation between tumor tissues PR expression and age (P >0.05). There was no correlation between tumor tissues ER, PR expression and sex, staging, tumor location, size, gross and histological type, invasive depth, l

关 键 词：结直肠肿瘤 雌激素受体 孕激素受体 受体放射配 基结合分析法 

分 类 号：R735.34[医药卫生—肿瘤]