c-fos反义寡脱氧核苷酸对茁-淀粉样蛋白31~35神经毒作用的影响  

Effects of antisense oligodeoxynucleotide against c-fos gene upon neurotoxicity of β-amyloid peptide fragment 31-35

作　　者：郑肇青[1] 张宇[1] 乔健天[1] 

机构地区：[1]山西医科大学神经生物学研究室,030001

出　　处：《中国药物与临床》2004年第6期437-439,共3页Chinese Remedies & Clinics

基　　金：山西省青年科学基金资助项目(20041044)

摘　　要：目的研究c-fos基因对β-淀粉样蛋白31～35神经毒的作用.方法培养原代小鼠皮层神经元,提前加入不同浓度的c-fos反义寡脱氧核苷酸,以DNA电泳分析和流式细胞技术检测其对β-淀粉样蛋白31～35致凋亡作用的影响.结果低浓度的c-fos反义寡脱氧核苷酸对β-淀粉样蛋白31-35所引起的神经元凋亡有保护作用.结论c-fos途径是β-淀粉样蛋白31-35神经毒作用的必需途径.Objective To explore whether the increased expression of c-fos gene is indispensable in the AβP31~35-induced apoptotic processes.Methods Pretreatment of cultured neurons with specific antisense oligod-eoxynucleotide (ASO) against c-fos was done, and the apoptosis-inducing action of AβP31~35 was examined by using eletrophoretic techniques, flow-cytometric assay.Results ASO against c-fos exhibited a neuroprotective effect upon the apoptosis induced by AβP31~35.Conclusion It is suggested that the expression of c-fos is pivotal for the AβP31~35-induced neuronal apoptosis.

关 键 词：C-FOS 反义寡脱氧核苷酸 Β-淀粉样蛋白 神经毒作用 ALZHEIMER病 

分 类 号：R749.1[医药卫生—神经病学与精神病学]