胰腺癌组织中IL-8 mRNA,TSP-1 mRNA表达及与微血管计数的关系  

Study on expressions of IL-8 mRNA, TSP-1 mRNA and their relationship with microvessel counts in pancreatic carcinoma tissues

作　　者：黄生福[1] 杨竹林[1] 苗雄鹰[1] 王群伟[1] 

机构地区：[1]中南大学湘雅二医院肝胆疾病研究室,湖南长沙410011

出　　处：《中国现代医学杂志》2004年第12期83-85,共3页China Journal of Modern Medicine

摘　　要：目的研究胰腺癌IL-8mRNA,TSP-1mRNA表达及其促或抑胰腺癌肿瘤血管生成的作用。方法54例胰腺癌手术切除标本经10%中性福尔马林固定后常规制作石蜡包埋切片,MV染色为常规ABC免疫组化法,IL-8mRNA和TSP-1mRNA染色为原位杂交染色法。结果54例胰腺癌IL-8mRNA和TSP-1mRNA阳性率分别为59.3%和55.6%,MVC为(64.2±11.8)个/HP。高分化腺癌和未转移病例MVC和IL-8mRNA阳性率明显低于中或低分化腺癌及转移病例,均有显著或高度显著差异(<0.05或<0.01),但TSP-1mRNA表达则相反。IL-8mRNA阳性病例和TSP-1mRNA阴性病例MVC明显高于IL-8mRNA阴性病例和TSP-1mRNA阳性病例,均有高度显著差异(<0.01)。IL-8mRNA与TSP-1mRNA表达呈密切负相关(χ2=7.1,<0.01)。结论IL-8mRNA和TSP-1mRNA表达与胰腺癌血管生成密切相关,IL-8具有促血管生成作用,而TSP-1具有抑血管生成作用。Objectives: To study the expressions of IL-8 mRNA, TSP-1 mRNA and their effects on the promoting or inhibiting angiogenesis of pancreatic carcinoma tissues. Methods: The assays of IL-8 mRNA, TSP-1 mRNA were detected by in situ hybridization techniques and the microvessel counts (MVC) was completed by immunohistochemical method of avidin-biotin complex on the 10% neutral formalin-fixed and routinely paraffin-embedded sections of 54 cases with pancreatic carcinoma. Results: The positive rate of IL-8 mRNA and TSP-1 mRNA was 59.3% and 55.6% respectively. The MVC was (64.2±11.8)/HP. The positive rates of IL-8 mRNA in the well-differentiated cases without metastasis were significantly lower than that in mediately or poorly-differentiated ones with metastasis, but that of TSP-1 mRNA was opposite. The MVC in the positive cases of IL-8 mRNA or the negative cases of TSP-1 mRNA was significantly higher than that in the negative ones of IL-8 mRNA or the positive ones of TSP-1 mRNA. The closely negative correlation was found between the expressions of IL-8 mRNA and TSP-1 mRNA. Conclusions: The expressions of IL-8 mRNA and TSP-1 mRNA might be closely related to the angiogenesis of pancreatic carcinoma tissues, IL-8 might have the promoting effect and TSP-1 might have inhibiting effect on the angiogenesis of malignant lesions.

关 键 词：胰腺肿瘤 血管生成 细胞因子类 微血管 信使RNA 免疫组织化学 原位杂交 

分 类 号：R736.7[医药卫生—肿瘤]