墨西哥香荚兰染色体及其变异的研究初报  

A primary study on chromosome and its variation of Vanilla planifolia Andr.

作　　者：陈小兰[1] 邓攀博[1] 杨明挚[1] 周恒苍 陈善娜[1] 

机构地区：[1]云南大学生物技术系,云南昆明650091 [2]云南省香荚兰公司,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2004年第4期360-363,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省科技"九五"攻关项目(95A2-8);"211工程"二期建设费及植物学博士点经费资助.

摘　　要：对栽培品种墨西哥香荚兰(VanillaplanifoliaAndr.)进行了常规染色体分析,并对其杂交品种、无性繁殖植株(组培苗、扦插苗)和野生品种滇南香荚兰(V.siamensisRolfeexDownie)的染色体变异进行了观察比较.结果表明,香荚兰染色体的大小在0.2～2μm之间,为小染色体,2N=28,经长期继代培养的组培苗染色体出现变异的频率明显超过扦插苗和野生品种,微核、染色体桥和染色体断片的频率明显增高.Chromosome of Vanilla planifolia Andr.were studied in this paper.Chromosome abnormity of asexual reproduction (including tissue culture and cuttage)plants of Vanilla planifoliar were observed and compared with its hybrid breed and wild breed Vanill siamensis Rolfe ex Downie.The results showed that V.planifolia have 28 small chromosomes whose size ranges from 0.2 to 2 micron.The frequency of chromosome abnormity of tissue culture plants was higher than that of cuttage and wild plants.It's frequency of micronuclei,chromosome bridge and chromosome lag apparently increased.

关 键 词：墨西哥香荚兰 染色体变异 滇南香荚兰 微核 染色体桥 染色体断片 兰科 有丝分裂 

分 类 号：Q949.718.4[生物学—植物学] Q343.2