人心肌铜锌超氧化物歧化酶的免疫组化定位  

Inununocytochemical Localization Study of Copper-Zinc Superoxide Dismutase in Human Myocardium

作　　者：郑毅 刘振华[2] 蔡凯华 

机构地区：[1]海军总医院普通内科,100037 [2]第二军医大学病理解剖学教研室,200433 [3]长海医院病理科,200433

出　　处：《第二军医大学学报》1993年第3期229-232,共4页Academic Journal of Second Military Medical University

摘　　要：用光镜免疫组化和免疫电镜技术观察了8例正常人心肌中Cu-Zn SOD的亚细胞定位,并用放射免疫法测定了心肌中Cu-Zn SOD的含量。结果显示多数心肌细胞含有Cu-Zn SOD,心室肌Cu-Zn SOD含量略高于心房肌。Cu-Zn SOD金标记颗粒分布于胞浆及核中,线粒体中略少,溶酶体中仅有极少量分布,肌浆网和高尔基器等膜结构上几乎未见Cu-Zn SOD金标记颗粒。本研究的结果表明,心肌是Cu-Zn SOD含量较丰富的组织。The distribution of copper-zinc superoxide dismutase (Cu-ZnSOD) in subcellular organelles in 8 cases of normal human myocardium was studied by using immunocytochemistry and immunoelectron microscopic technique and the contents of Cu-Zn SOD in myocardium were measured by radioimmunoassay. The results showed that Cu-ZnSOD was in most myocardial cells. The contents of Cu-ZnSOD in the ventricular myocardial cells were higher than those in the atrial myocardial cells. The Cu-ZnSOD labeled with immunogold particles were distributed throughout cytoplasm and nuclei, but less in mitochondria and a very little in lysosomes. No gold particle was found on the membranous structure of sarcoplasmic reticulum as well as Golgi complex. The results demonstrate that the myocardium was the tissue containing quite abundant Cu-Zn SOD.

关 键 词：心肌 超氧物歧化酶 免疫组织化学 

分 类 号：R361.3[医药卫生—病理学]