毛细管气相色谱法测定芬太尼的血药浓度  

Capillary GC Determination of Fentanyl in Human Plasma

作　　者：刘垣升[1] 吴玉田[1] 孔庆洪 王新华 

机构地区：[1]第二军医大学药学院药物分析教研室,第二军医大学教学保障处200433 [2]长征医院麻醉科,200433

出　　处：《第二军医大学学报》1993年第6期517-520,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金

摘　　要：本文报告了用气相色谱(GC)法测定人体血浆中芬太尼含量的方法。以Ro21-2212作内标,芬太尼的同系物作载体,在碱性条件下用环己烷-异戊醇(197:3)提取,药物反提至硫酸溶液中,碱化后再用乙醚-二氯甲烷(9:1)提取。样品存柱温为255℃的HP-1大口径毛细管柱(10m×0.53mm×2.65μm)上,以氮气作载气和氮磷检测器进行分析。住0.25～100ng/ml范围内有良好线性,最低检测限为0.2ng/ml。平均回收率为99.02％±6.81％,日内和日间变异系数均小于8.0％。方法重现性好、专一性强、灵敏度高,内源性物质、代谢产物及同时使用的药物均不干扰。用本法测定了全麻择期手术病人的药代动力学参数。A GC method is described for measuring fentanyl in man plasma. Fentanyl was extracted at basic pH with cyclohexane-isobutyl alcohol (197:3) with Ro21-2212 added as internal standard and fentanyl derivative as earner. The drug was back-extracted in H2SO4, then the extract was made basic and recxtracted with ethyl ether-dichloromethane (9:1). The organic phase was evaporated at 40℃ under N2 and the residue was dissolved in ethanol. A. portion (2μl) was analyzed on a wide-bore capillary column (10 m × 0.53 mm) of HP-1 (2.65 μm), operated at 255℃ with N2 as carrier gas (8 ml/min) and N -P detector. The calibration graph was linear within the ranges of 0.25-100 ng/ml with a corelation coefficient of 0.9996 and the detected limit was 0.2 ng/ml. Mean recovery was 99.02% ± 6.81%. The coefficient of variation for the within- and between-run were all less than 8%. No interference was found from endogenous compounds, metabolites of parent drug or other commonly used drugs. The method was applied to therapeutic drug monitoring and pharmacokinetic studies.

关 键 词：芬太尼 气相色谱法 药物动力学 

分 类 号：R914.1[医药卫生—药物化学]