人原发性肝细胞癌及肝硬变内HBV DNA及HBAg的分布及意义  

Distribution and significance of HBV DNA and HBAg in human primary hepatocellular carcinoma and liver cirrhosis

作　　者：韩慧霞[1] 王文亮[1] 隋延仿[1] 朱新生[1] 

机构地区：[1]第四军医大学病理学教研室

出　　处：《第四军医大学学报》1993年第5期367-371,共5页Journal of the Fourth Military Medical University

摘　　要：为了探讨HBV与肝细胞癌的发病的关系,作者应用原位分子杂交,免疫组化及它们的双标记方法在44例肝癌,17例癌旁肝组织和42例肝硬变的石蜡切片中检测了HBV DNA,HBxAg和HBcAg。研究结果显示,HBV DNA,HBxAg和HBcAg在肝癌组织中的检出率分别为70.5％,65.9％和 20.5％,而在癌旁肝组织内检出率分别为76.5％,76.5％和29.4％,42例肝硬变组织中的检出率分别为88.1％,69.0％和33.3％。本研究中癌周肝组织和肝硬变组织中,HBV DNA,HBxAg和HBcAg的检出率高于肝癌组织,但无统计学意义。本研究结果表明肝细胞肝癌的发生与HBV慢性持续性感染有密切关系。In order to explore the relationship between HBV and hepatocellular carcinoma (HCC), hepatitis B virus DNA (HBV DNA), HBxAg and HBcAg were detected in paraffin-embedded sections of 44 cases of HCC, 17 cases of cancerous hepatic tissue and 42 cases of liver cirrhosis (LC) by in situ hybridization (ISH), immunohistochemical (IHC) assay and its double labelling technique. The results showed that the detactabili-ty rates of HBV DNA, HBxAg and HBcAg in cancerous tissues were 70. 5%, 65. 9% and 20.5%, respectively, and that of HBV DNA, HBxAg and HBcAg around cancerous tissues were 76. 5%, 76. 5% and 29. 4% respectively. Among the 42 cases of LC, the detection rates of HBV DNA, HBxAg and HBcAg were 88. 1% , 69. 0% and 33. 3% respectively. In this study, the detection rates of HBV DNA, HBxAg and HBcAg around cancerous tissues and in LC were higher than that in hepatoma tissues, but the difference was not statistically significant (F>0. 05). The results of this study suggestd that a relationship between pathogenesis of HCC and chronic persistent infection of HBV is very close.

关 键 词：肝细胞癌 分子杂交 肝硬变 原发性 

分 类 号：R735.7[医药卫生—肿瘤]