中国精神病人外周血博尔纳病病毒P24基因片段检测  

Detection of the Borna disease virus P24 gene fragement in peripheral blood of patients with psychiatric disorders in China

作　　者：徐平[1] 谢鹏[1] 邹德智[1] 左联[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016

出　　处：《中国人兽共患病杂志》2004年第6期471-473,476,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目(No:39870252)

摘　　要：目的 探讨博尔纳病病毒与人类精神疾病的关系。方法 采用套式逆转录酶聚合酶链反应(nested RT-PCR)结合荧光定量(FQ)PCR检测了31例精神分裂症病人,16例情感障碍病人以及50例健康人外周血单个核细胞(PBMC)中BDV P24基因片段。结果精神分裂症组BDV P24基因片段阳性率为9.7％(3/31);情感障碍组(0/16)和健康组(0/50)均为阴性。精神分裂症组阳性率高于健康组,但差异无显著性意义(P>0.05)。结论 中国的精神病人中存在BDV感染,但BDV感染是否与精神分裂症发病相关有待进一步研究。To investigate the relationship between Borna disease virus (BDV) and the human psychiatric disorders and the frequency of the BDV infection in China. The P24 fragments of BDV RNA in peripheral blood mononuclear cells (PBMC) from 31 patients with schizophrenia, 16 patients with affective disorders and 50 healthy donors were detected by nested RT-PCR and fluorescence quantitative (FQ) PCR.The results showed that the positive rate of the P24 fragment of BDV RNA in PBMC from patients with schizophrenia( 9.7%) was higher than that of patients with affective disorders (0 %) and of healthy donors (0 % ), but the differences did not reach statistical significance. These results suggest that BDV infection exists in the psychiatric patients in China. Further study is necessary to clarify the possible association between BDV infection and the onset of human psychiatric disorders.

关 键 词：精神分裂症 情感障碍 博尔纳病病毒 套式逆转录酶聚合酶链反应 荧光定量 检测 

分 类 号：R749.3[医药卫生—神经病学与精神病学]