分次大剂量L-精氨酸腹腔内注射致小鼠急性水肿性胰腺炎模型的研究  

Study on the model of acute pancreatitis induced by of twice intraperitoneal injection excessive doses of L-arginine in mice

作　　者：郑吉顺[1] 陈垦[2] 王晖[3] 林振和[3] 

机构地区：[1]合肥市第一人民医院消化科,合肥230061 [2]广东药学院临床医疗系,广州510224 [3]广东医学院药理教研室,湛江524023

出　　处：《中国药理学通报》2004年第6期707-710,共4页Chinese Pharmacological Bulletin

基　　金：广东省教育厅自然科学研究项目;NoZ0 2 0 44

摘　　要：目的　建立一种操作简单、病变典型、稳定性好的小鼠急性水肿性胰腺炎 (AEP)模型。方法　小鼠分次大剂量L 精氨酸腹腔内注射 (2× 2 0mg·g-1) ,注射后 6、12、2 4、4 8、72h观察血清淀粉酶水平、胰腺病理学改变及胰腺组织湿 /干重量比率的变化。结果　注射后 6h即出现典型的急性水肿性胰腺炎病理改变 ,2 4h达高峰 ,4 8h出现少量胰腺细胞坏死 ,72h病变减轻 ;胰腺组织湿 /干重量比率变化、病理评分呈同样的时相性变化。血清淀粉酶 6h时显著增高 ,2 4h达高峰 ,为对照组的 4～ 5倍 ,72h接近正常水平。结论　分次大剂量L 精氨酸腹腔内注射诱导的小鼠胰腺病变符合典型的急性水肿性胰腺炎病理学改变 ,是较为理想和值得推广的动物模型。AIM To establish a new, simple, stable and classical experimental model of acute edematous pancreatitis. METHODS Male mice were injected intraperitoneally 2×200 mg·(100 g) -1 body weight of L-arginine in an 1 h interval, as a 20% solution in 0.15 mol·L -1 NaCl. Control mice received the same quantity of 0.15 mol·L -1 NaCl. The mice were killed at 6, 12, 24, 48, 72 h following L-arginine administration. The serum amylase level, wet/dry weight ratio of the pancreas, histologic were assessed. RESULTS The serum level of amylase was significantly elevated at 6 h,reached the peak level at 24 h, normalized at 72 h. Histologic examination revealed interstitial edema and inflammatory cell infiltration reached the peak level at 24 h and decreased at 48,72 h. The wet/dry weight ratio of the pancreas changed in accordance with the interstitial edema. CONCLUSIONS The present study has demonstrated that the administration of excessive doses of arginine induces a new, noninvasive experimental model of acute edematous pancreatitis.

关 键 词：小鼠 L-精氨酸 急性水肿性胰腺炎 动物/模型 

分 类 号：R-332[医药卫生] R329.24