rhBMP-2m对辐射小鼠骨髓CD34^+细胞变化的影响  

The effect of rhBMP-2m on the CD34^+ cells in mouse bone marrow after irradiation

作　　者：滕增辉[1] 田琼[1] 梅其炳[1] 曹大勇[1] 杨静悦[1] 杨文涛[1] 李康樗[1] 

机构地区：[1]第四军医大学基础部药理学教研室,西安710032

出　　处：《中国药理学通报》2004年第6期619-622,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目;No 3 9970 2 31

摘　　要：目的　探讨小鼠受到辐射后 ,重组人骨形成蛋白成熟肽 2 (rhBMP 2m)对骨髓CD34+ 细胞变化的影响。方法　通过60 Coγ射线照射 ,建立小鼠骨髓造血损伤模型 ,经rhBMP 2m连续治疗后 ,骨髓单个核细胞计数 ,通过流式细胞仪检测骨髓单个核细胞中CD34+ 细胞比例 ,比较对照组和治疗组之间的差别。结果　经rhBMP 2m治疗的动物 ,单个核细胞数和CD34+ 细胞比例均明显高于照射对照组 ,P <0 0 5 ,n=6。结论　对于辐射引起的小鼠骨髓造血损伤 ,rhBMP 2m能够增加造血细胞数量 ,提高骨髓单个核细胞中CD34+ 细胞的比例 ,加快骨髓造血系统的重建。AIM To observe the effect of rhBMP-2m on the CD34+ cells in mouse bone marrow and discuss the role of rhBMP-2m in the process of hematopoietic reconstitution after irradiation. METHODS The animal model of bone marrow hematopoietic injury was established by irradiation with 60Coγ radial resource; the number of mononuclear cells were counted using hemocytometer and the percentage of CD34+ cells in bone marrow mononuclear cells(BMMNC) was detected by Flow Cytometric after therapy with rhBMP-2m. RESULTS ①The number of mononuclear cells and the percentage of CD34+ cells decreased as compared with the normal group after irradiation;②The number of mononuclear cells and the percentage of CD34+ cells were more than irradiation group after therapy with rhBMP-2m. CONCLUSIONS For the bone marrow hematopoietic injury of mouse induced by irradiation, rhBMP-2m can increase the number of mononuclear cells and the percentage of CD34+ cells, accelerate the reconstructed process of hematopoiesis at the same time.

关 键 词：辐射损伤 RHBMP-2M CD34 

分 类 号：R-332[医药卫生] R329.24