腺病毒介导mIFN-β基因转染的小鼠黑色素瘤细胞体内致瘤性及抗肿瘤作用的实验研究  

Study of in vivo tumorigenicity and anti-tumor effects of adenovirus-mIFN-β transfected murine melanoma cells

作　　者：吴云[1] 徐容[1] 曹广文[2] 潘卫[2] 陈秋莉[2] 沈毅君 卢海妹[1] 蔡春晓[1] 邓松华[1] 

机构地区：[1]安徽医科大学病理生理学教研室,合肥230032 [2]第二军医大学微生物教研室,上海200433 [3]复旦张江生物医药股份公司,上海200000

出　　处：《中国药理学通报》2004年第6期677-680,共4页Chinese Pharmacological Bulletin

基　　金：安徽省教育厅自然科学基金资助课题;No 2 0 0 1kj14 3

摘　　要：目的　研究腺病毒 (adenovirus,Ad)介导mIFN β基因转染的B16小鼠黑色素瘤细胞的体内致瘤性及抗肿瘤作用。方法　用携带mIFN β基因的重组腺病毒载体体外转染B16细胞 ,将该细胞接种于小鼠 ,观察其在小鼠体内的致瘤性及对野生型B16细胞的致瘤性有无影响。结果　经腺病毒介导 ,mIFN β基因成功地导入B16细胞并获得有效表达 ;转mIFN β基因的B16细胞的体内致瘤性明显降低 ,并能抑制对侧野生型B16细胞的致瘤性。结论　该研究结果提示利用腺病毒载体携带IFN β基因来开发瘤苗治疗肿瘤具有良好的应用前景。目的　研究腺病毒 (adenovirus,Ad)介导mIFN β基因转染的B16小鼠黑色素瘤细胞的体内致瘤性及抗肿瘤作用。方法　用携带mIFN β基因的重组腺病毒载体体外转染B16细胞 ,将该细胞接种于小鼠 ,观察其在小鼠体内的致瘤性及对野生型B16细胞的致瘤性有无影响。结果　经腺病毒介导 ,mIFN β基因成功地导入B16细胞并获得有效表达 ;转mIFN β基因的B16细胞的体内致瘤性明显降低 ,并能抑制对侧野生型B16细胞的致瘤性。结论　该研究结果提示利用腺病毒载体携带IFN β基因来开发瘤苗治疗肿瘤具有良好的应用前景。AIM To observe the tumorigenicity and anti-tumor effects of adenovirus-mIFN-β transfected murine melanoma cells B16. METHODS The B16 cells were transefcted by recombinant adenovirus carrying mIFN-β gene in vitro, then the tumorigenicity and anti-tumor effects of AdmIFN-β transfected B16 cells in vivo was observed.RESULTS mIFN-β gene was successfully transduced into B16 cells mediated by adenovirus and expressed effectively. mIFN-β transduced into B16 cells mediated by adenovirus not only resulted in an efficient inhibition of tumorigenicity in situ but also led to a growth inhibition of parental cells at other sites. CONCLUSIONS This study indicates that it has bright prospect to develop tumor vaccine and treat tumor by using adenovirus vector harboring IFN-β gene.

关 键 词：干扰素-Β 瘤苗 腺病毒 黑色素瘤 

分 类 号：R332[医药卫生—人体生理学] R329.2[医药卫生—基础医学]