VEGF及其受体KDR在宫颈癌的联合表达与局部肿瘤血管生成的关系  

The Association of Expression of Vascular Endothelial Growth Factor and Its Receptor,Kinase Insert Domain Containing Receptor with Tumor Angiogenesis in Invasive Carcinoma of Cervix

作　　者：江忠清[1] 朱凤川[1] 曲军英[1] 郑秀[1] 张彬[1] 吴桂珠[1] 

机构地区：[1]福建医科大学附属第一医院妇产科,福州市350005

出　　处：《中国肿瘤临床》2004年第12期671-674,共4页Chinese Journal of Clinical Oncology

基　　金：福建省教育厅科研基金资助(编号:01B017)

摘　　要：目的:探讨血管内皮生长因子及其受体KDR在早期宫颈癌的表达及其对宫颈癌肿瘤血管生成的作用。方法:采用免疫组织化学SP法检测18例宫颈上皮内瘤样病变(cervicalintraepithelialneoplasm,CIN)、75例早期宫颈癌(invasivecarcinomaofcervix,ICC)和15例正常宫颈上皮(normalcervicalepithelium,NCE)中VEGF和KDR的表达情况,并检测其中微血管密度(CD34标记)。结果:在ICC中,VEGF和KDR主要表达于癌细胞膜和(或)细胞浆;而CD34主要表达于癌巢间质血管内皮细胞。从NCE到CIN再到ICC,VEGF与KDR的阳性表达率以及MVD均显著升高(P<0.01)。在ICC中,VEGF、KDR阳性表达者其MVD分别显著高于VEGF、KDR阴性表达者(P<0.05)。VEGF在ICC的表达与KDR显著正相关(r=0.56,P<0.01),并且两者均与MVD显著正相关(前者r=0.60,P<0.01;后者r=0.33,P<0.01)。VEGF与KDR均阳性表达者,其微血管密度显著高于两者均阴性表达者(P<0.01)。结论:VEGF及其受体KDR表达在宫颈癌肿瘤血管生成中起上调作用,两者均过度表达,肿瘤血管生成显著增加。检测VEGF及其受体KDR的联合表达对进一步了解宫颈癌血管生成情况以及寻找抗肿瘤血管生成治疗新靶点有一定价值。Objective: The expression of vascular endothelial growth factor (VEGF), and its receptor, kinase insert domain containing receptor (KDR) and its significance in regulating tumor angiogenesis in early invasive carcinoma of cervix was investigated. Methods: Expression of VEGF, and KDR of cancer cells and microvessel density (MVD) in tumor stroma labeled by CD34 in 18 cases of cervical intraepithelial neoplasm (CIN), 75 cases of early invasive carcinoma of cervix (ICC) and 15 cases of normal cervical epithelium (NCE) were detected by immunohistochemistry SP method. Results: In ICC, VEGF and KDR was mainly expressed in the cellular membrane and/or cytoplasm of tumor cells, whereas expression of CD34 was mainly in the vascular epithelial cells of tumor stroma. The positive rate of VEGF and KDR, and MVD increased remarkably from NCE to CIN, and then to ICC, accordingly (P<0.01). In the cases with positive expression of VEGF, and KDR in ICC, the MVD was significantly higher than that in the cases with negative expression of VEGF, and KDR, respectively (P<0.05). In ICC, the MVD was positively related to the expression of VEGF (r=0.60, P<0.01), and to KDR (r=0.33, P<0.01), respectively. The expression of KDR was also positively related to VEGF (r=0.56, P<0.01). In the cases with VEGF and KDR being both positively expressed, the MVD was remarkably higher than that in the cases with both negative expression of VEGF and KDR (P<0.01). Conclusions: Expression of VEGF and its receptor KDR may play an important role in up-regulating tumor angiogenesis in cervical carcinoma. Overexpression of both VEGF and KDR in ICC may result in rapid tumor angiogenesis. Detection of co-expression of VEGF and KDR may be of value in further understanding tumor angiogenesis and searching new target for anti-angiogenesis therapy in invasive carcinoma of cervix.

关 键 词：宫颈肿瘤 血管内皮生长因子 血管内皮生长因子受体KDR 肿瘤血管生成 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]