复发直肠癌切端的细胞增殖动力学分析  

An Analysis of Cell Proliferation Dynamics of Relapse Rectum Carcinoma Incisal Margin

作　　者：耿敬姝[1] 宋鸿涛[1] 王艳颖[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院病理科,哈尔滨市150040

出　　处：《中国肿瘤临床》2004年第12期661-663,共3页Chinese Journal of Clinical Oncology

基　　金：黑龙江省自然科学基金资助(编号:D9820)

摘　　要：目的:对60例直肠癌Dixon术后切端组织的细胞增殖状态进行研究,旨在探讨细胞增殖动力学在Dixon术后复发中的作用。方法:切取石蜡包埋组织,加入二甲苯脱蜡,梯度乙醇脱苯。水化后加入胃蛋白酶消化,过滤后离心,弃上清进行碘化丙啶染色,应用流式细胞术(FCM)对直肠癌组织及切端的DNA指数(DI)、S期细胞百分比(SPF)、细胞增殖指数(PI)进行检测。结果:未复发组切端DI为1.09±0.03,SPF为4.18±1.32,PI为8.69±2.13。复发组切端DI为1.23±0.11,SPF为10.98±3.12,PI为14.13±3.67。经统计学t检验复发组与未复发组切端两组间SPF及PI均可见显著性差异(P<0.01)。结论:细胞增殖动力学可作为大肠癌复发的一个重要因素。手术范围除考虑肠壁内浸润距离外,还应包括切除全部癌周围高增殖活性的粘膜组织。Objective: The inadequate range of operation excise is one of the main reasons of rectum carcinoma part relapse after operation. To elucidate the role of cell proliferation dynamics in the relapse after operation we examined the state of cell proliferation dynamics of 60 rectum carcinoma incisal margin tissues after Dixon operation. Methods: The carcinoma incisal margin tissues embedded in paraffin were sectioned, dewaxing in xylene, dedenzened in gradually ethanol. After rehydrated, the sections were digested with pepsin, filtered, centrifuged, discarded the suspension and dyed with propidium iodide. To examine the DNA index (DI), Synthetic phase fraction (SPF), Proliferative index (PI) of carcinoma tissues and incisal margin tissues with Flow cytometry (FCM). Results: In no-relapse group carcinoma incisal margin tissues, DI is 1.09±0.03, SPF is 4.18±1.32, PI is 8.69±2.13 respectively. In relapse group carcinoma incisal margin tissues, DI is 1.23±0.11, SPF is 10.98±3.12, PI is 14.13±3.67 respectively. By the t test analysis, SPF and PI showed highly significant difference between relapse and no-relapse group (P<0.01). Conclusion: The cell proliferation dynamics may act as a important factor of rectum carcinoma relapse. The range of operation should consider both the distance of carcinoma infiltrate in intestinal wall and all high proliferation mucosa tissue of peripheral carcinoma tissues.

关 键 词：直砀癌 切端 DNA指数 细胞增殖指数 细胞增殖动力学 

分 类 号：R735.37[医药卫生—肿瘤]