转化生长因子-茁1在人横纹肌肉瘤肌源性分化中的作用  

Role of Transforming Growth Factor-in Myogenic Differentiation of Human Rhabdomyosarcoma

作　　者：王华[1] 杨光华[1] 步宏[1] 郭立新[1] 周桥[1] 李胜富[2] 彭惠[3] 

机构地区：[1]四川大学华西医院病理科 [2]四川大学华西医院移植免疫实验室 [3]重庆医科大学病理学教研室

出　　处：《中国肿瘤临床》2004年第12期705-708,共4页Chinese Journal of Clinical Oncology

基　　金：美国纽约中华医学基金会(CMB)基金项目资助(编号:00-722)

摘　　要：目的:探讨转化生长因子-茁1(TGF-茁1)在横纹肌肉瘤肌源性分化中的作用。方法:采用细胞培养、逆转录多聚酶链式反应(RT-PCR)及免疫荧光染色观察TGF-茁1对横纹肌肉瘤细胞系RD肌源性分化相关指标的影响,并用免疫组化SP法,检测49例横纹肌肉瘤组织中TGF-茁1表达与肌球蛋白重链(MyHC)之间的相关性。结果:TGF-茁1可明显抑制RD细胞肌球蛋白重链mRNA和蛋白的表达。用TGF-茁1处理RD细胞后,肌球蛋白重链和肌节肌动蛋白(Sarcomeric-Actin)阳性着色细胞数明显下降(P<0.05)。MyoD1的表达在处理前后未见明显差异。横纹肌肉瘤组织中TGF-茁1表达与肌球蛋白重链呈明显负相关(P<0.05)。结论:TGF-茁1可抑制RD细胞的肌源性分化,这一作用可能不依赖于MyoD1的调节。TGF-茁1信号传导通路可能与横纹肌肉瘤的肌源性分化受阻有关。Objective: To evaluate the effect of transforming growth factor- (TGF- in myogenic differentiation of human rhabdomyosarcoma (RMS). Methods: Myogenic differentiation was analyed as the mRNA level of myosin heavy chain (MyHC) by reverse transcription-polymerase chain reaction (RT-PCR). Immunofluorescent staining was used to detect the expression of MyHC, Sarcomeric-Actin and MyoD1. Immunohistochemistry was used to detect the expression of TGF- and MyHC in 49 cases of rhabdomyosarcoma. Results: Exogenous TGF- downregulated expression of MyHC mRNA. Moreover, treatment with TGF- significantly decreased the number of MyHC and Sarcomeric-Actin-expressing cells when compared with the control (P<0.05). However, TGF- treatment didn't change the expression of MyoD1. There was a close negative correlation between the expression of TGF- and MyHC in RMS tissues (r=-0.661, P<0.05). Conclusion: TGF- may inhibit myogenic differentiation independent of its effects on MyoD1 in RD cells. TGF- signal transduction may play an important role in myogenic differentiation of RMS cells.

关 键 词：转化生长因子-Β1 横纹肌肉瘤 肌源性分化 免疫组化SP法 

分 类 号：R738.7[医药卫生—肿瘤]