丹参含药血清对肝星状细胞增生的抑制作用  

Inhibitory effect of Danshen-containing serum on proliferation of HSC-T6 cells

作　　者：谈博[1] 张奉学[1] 刘妮[1] 郭兴伯[1] 

机构地区：[1]广州中医药大学热带医学研究所,广东省广州市510405

出　　处：《世界华人消化杂志》2004年第6期1357-1360,共4页World Chinese Journal of Digestology

基　　金：广东省自然科学基金资助项目;No.000347~~

摘　　要：目的:研究丹参含药血清对肝星状细胞(HSC)的增生抑制作用,并探讨中药抗肝纤维化筛选平台的建立. 方法:测定HSC-T6细胞的细胞生长曲线和克隆形成率,观察其细胞增生状况.取原始血清体积分数的80%,40%, 20%,10%,5%的丹参含药血清作用于HSC-T6细胞, 观察其增生抑制效应及量效关系. 结果:HSC-T6细胞的细胞群体倍增时间为10.57 h,克隆形成率为82.4%,说明该转化细胞系的活力较好,增生能力较强.在5-80%浓度范围之内,丹参含药血清抑制HSC细胞增生作用呈剂量依赖性(经直线回归分析,相关系数r=0.9487). 结论:丹参含药血清能明显抑制HSC-T6细胞增生,呈剂量依赖性.AIM: To investigate the inhibitory effect of Danshen-con-taining serum on the proliferation of HSC-T6 cells and to construct anti-hepatic-fibrosis in vitro system for screening potential herbal drugs. METHODS: The proliferation status of HSC-T6 cells was evaluated by calculating its growth curve and cloning efficiency. HSC-T6 cells were exposed to different volume fractions of Danshen-containing serum, including 80%, 40%, 20%, 10%, and 5% of original serum, and its inhibitory effect and dose-effect relationship were investigated. RESULTS: The population doubling time of HSC-T6 was 10.57 hours and its cloning efficiency was 82.4%, which showed that this cell line had good capacity of activity and proliferation for tests. Ranging from 5% to 80% of original concentration, Danshen-containing serum had the dose-dependent inhibitory effect on HSC-T6 cell (analysis by linear regression, coefficient of correlation=0.9 487). CONCLUSION: The Danshen-containing serum has significantly inhibitory effect on proliferation of HSC-T6 cells in a dose-dependent manner.

关 键 词：丹参含药血清 肝星状细胞 细胞增生 抑制作用 HSC 血清浓度 

分 类 号：R285.5[医药卫生—中药学]