嘌呤霉素肾病大鼠肾小球足细胞相关分子表达的共定位分布  

Colocalization of podocyte-associated molecules in puromycin aminonucleoside nephrotic rats

作　　者：邓江红[1] 丁洁[1] 管娜[1] 范青锋[1] 张敬京[1] 

机构地区：[1]北京大学第一医院儿科,北京100034

出　　处：《肾脏病与透析肾移植杂志》2004年第3期205-210,共6页Chinese Journal of Nephrology,Dialysis & Transplantation

基　　金：国家自然科学基金资助项目 (No :3 0 170 992 ) ;国家教育部留学回国人员科研启动金资助项目 ( 2 0 0 3 -14 )

摘　　要：目的 :研究嘌呤霉素肾病大鼠肾小球足细胞相关分子在病变过程中的表达与分布的关系及变化。　 方法 :4 2只SD大鼠随机分为实验组和对照组 ,建立嘌呤霉素肾病模型 ,并在 2 4h、36h、2天、5天、1 0天、1 5天、2 0天七个不同的时间点 ,应用免疫荧光双标记的方法 ,通过激光共聚焦显微镜对足细胞相关分子nephrin、podocin ,α actinin 4以及CD2AP两两之间的分布关系及变化进行研究。　 结果 :正常大鼠肾小球中CD2AP与nephrin及podocin的染色沿肾小球血管襻有部分重叠 ,而两者的分布模式略有不同 ,但这种共定位关系不随病变的发展而变化 ;CD2AP与α actinin 4的分布也有部分重叠关系 ,且随着病变进展 ,其荧光重叠程度增加 ;α actinin 4与nephrin及podocin在正常时有极小部分重叠 ,随着病程变化 ,两者的分布模式与分布量的变化均不同步。　 结论 :足细胞相关分子nephrin、podocin及CD2AP之间以功能复合体的形式相互联系并参与蛋白尿的发生发展 。Objective:To study the expression and distribution of podocyte molecules in nephrotic rats induced by puromycin aminonucleoside (PAN), and to explore the possible interactions among these molecules in the development of proteinuria. Methodology:Forty two Sprague Dawley rats were enrolled in this study, they were randomly divided into experimental group (21 rats) and control group (21 rats). The minimal change nephropathy model was established by a single PAN injection in experimental group. While the control group received only 0.9% sodium chloride solution with the same dose. At the 24th hour, 36th hour, second, 5th, 10th, 15th and 20th day after injection, the rats were sacrificed and the kidneys were acquired. Double immunofluorescence was used to stain the pairs of molecules: CD2AP /nephrin, CD2AP/podocin, CD2AP/α actinin 4, α actinin 4/nephrin and α actinin 4/podocin. A laser scanning confocal microscopy was applied to study the distributions and the relationships of nephrin, podocin, α actinin 4 and CD2AP in the glomerular of the rats. Results:① In PAN nephrosis rats, the distribution of CD2AP was along the glomerular basement membrane with a segmental and linear pattern. Nephrin and podocin showed a similar leaner pattern along the capillary wall but not resembled CD2AP.② In normal rats, there was a partial co localization between nephrin and CD2AP as well as podocin and CD2AP, and their co localizations did not change with the development of the proteinuria. ③ α actinin 4 showed a weaker and granular staining pattern along the glomerular capillary wall in normal rats, but its staining was stronger in 20 days. There was also a little overlapping between the staining of CD2AP and α actinin 4. However, this interaction was stronger with the development of proteinuria, and then the interaction was weaker with the recovery of proteinuria. ④ At the same time, α actinin 4 was also detected to partly co localize with nephrin and podocin, but the degree of the co localization was

关 键 词：嘌呤霉素肾病 大鼠 肾小球 足细胞 分子表达 分子行为 

分 类 号：R726.91[医药卫生—儿科]