一种大鼠蒸气烫伤模型的建立  

Establishment of rat model of scalding with high pressure steam

作　　者：姚庆君[1] 贾赤宇[1] 陈璧[1] 汤朝武[1] 徐明达[1] 丁国斌[1] 王洪涛[1] 

机构地区：[1]第四军医大学西京医院烧伤科,西安710032

出　　处：《中华烧伤杂志》2004年第3期168-170,共3页Chinese Journal of Burns

摘　　要：目的　建立一种可控深度及面积的大鼠烫伤模型。　方法　用高压蒸气消毒锅及自制烫伤支架制作高压蒸气烫伤装置 ,用压力为 0 .12MPa(1MPa=75 0 0mmHg)、直径 2 .6cm的致伤孔上分别在大鼠背部烫 3、4、5、6、7、8、9、10s,每时相点 5个创面。伤后 2 4h取标本行组织学观察 ,用Photoshop软件测量烫伤深度 ,并观察有无毛囊、汗腺附件受损、烫伤区被毛生长及创面愈合情况。　结果　烫伤深度与烫伤时间的变化呈正相关 (r =0.99)。浅Ⅱ、深Ⅱ、Ⅲ度烫伤模型的致伤时间分别为 3、5、7s。烫伤 7～ 10s创面深度虽逐渐加重 ,愈合时间却相近。　结论　该模型可以控制烫伤深度、面积 ,烧伤深度划分准确且操作简便 ,是研究创伤修复机制及评价创面用药的较好模型。Objective To establish a rat model of scalding with controllable depth and area by high pressure steam. Methods High pressure steam apparatus consisting of an autoclave and a self-made scalding frame was employed in the study. The rats were inflicted with scalding with 0.12 Mpa (1Mpa=7500mmHg) high pressure steam on the back through a hole of 2.6cm in diameter for 3,4,5,6,7,8,9 and 10 seconds, with five wounds at each time point. The tissue samples were harvested at 24 post injury hour (PIH) for pathomorphological examination. The depth of scald was measured, and injury to the sweat gland and hair follicles injury, the hair growth in scalded area, and the wound healing condition were observed with Photoshop software. Results There was positive correlation between the scalding depth and scalding time. The injury time for superficial and deep partial thickness burn and full thickness burn were 3,5 and 7 seconds respectively. The wound healing time was similar even the scalding became more and more serious when injury time increased from 7 to 10 seconds. Conclusion The scalding depth and area in this model could be controlled, and the degree of scald injury could be graded accurately with easy manipulation. The result showed that it was an ideal model of skin burn wound.

关 键 词：烧伤 蒸气 动物模型 创伤 修复机制 

分 类 号：R-332[医药卫生]