一个新的产氢细菌的鉴定及产氢特性的研究  

Characterization of A New Clostridium Species and Its Hydrogen Production

作　　者：陈双雅[1] 东秀珠[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》2004年第4期411-416,共6页Acta Microbiologica Sinica

基　　金：中国科学院创新基金领域前沿项目资助~~

摘　　要：利用Hungate滚管技术从福建省漳州垃圾处理厂厌氧消化器的颗粒污泥中分离到一株产氢的细菌L1 5。菌株L1 5为严格厌氧的革兰氏阳性杆菌 ,菌体大小为 0 5 μm～ 0 7μm× 2 5 μm～ 5 0 μm ,以侧生鞭毛运动。在孢肉培养基上产生端生的卵圆形芽孢。温度生长范围 1 5℃～ 4 5℃ (最适温度 30℃～ 37℃ ) ;pH范围 5 0～ 8 4 (最适pH 6 3～6 8)。该菌株不水解明胶和七叶灵 ,不还原硫酸盐 ,牛奶变酸但不凝固 ,发酵多糖和少数的单糖、双糖和寡糖 ;发酵葡萄糖的最终产物为乙酸、丁酸、H2 和CO2 。G +C含量为 2 9 8mol%。 1 6SrDNA序列分析表明 ,该菌株属于梭菌的簇Ⅰ ,与Clostridiumparaputrificum较为接近 (相似性为 97 1 % )。通过生理特征和 1 6SrDNA序列的同源性分析 ,表明菌株L1 5应是梭菌属簇Ⅰ中的一个新种 ,命名为Clostridiumdefluvii。菌株L1 5保藏在中国普通微生物菌种保藏中心 ,保藏号为AS1 3489。菌株L1 5的最佳产氢温度为 34℃、pH为 7 0。当葡萄糖浓度为 0 4 %时 ,氢气产率可达到1 4 1molH2 mol葡萄糖。该菌可利用下列底物产酸产氢 ,括号内为产氢率 (底物浓度 1 % ) :果糖 (1 0 0molH2 mol)、麦芽糖 (2 1 7molH2 mol)、蔗糖 (1 6 9molH2 mol)、菊糖 (4 70molH2 mol)、糖原An obligately anaerobic, mesophilic, hydrogen producing Clostridium strain L15 was isolated from an upflow anaerobic sludge blanket (UASB) reactor treating manicipal sewage. The cells were Gram positive, spore forming, rod shaped. Growth was observed in the temperature range of 15℃～45℃ (optimum 30℃～37℃) and pH range of 5 0～8 4 (optimum pH 6 3～6 8). The strain fermented D fructose, glycogen, D glucose, inulin, maltose, melizitose, D raffinose, starch, sucrose, D trehalose, but did not ferment lactose, galactose and mannose.The end products formed from glucose were acetate, butyrate, carbon dioxide and hydrogen. Strain L15 hydrolyzed starch but not gelatin nor esculin. The G+C content of the strain was 29 8 mol%. Phylogenetic analysis based on the 16S rDNA sequence similarity indicated that the novel strain belonged to cluster I of the genus Clostridium, most close to Clostridium paraputrificum, with 97 1% similarity. Therefore, a new Clostridium species Clostridium defluvii was designated. Strain L15 produced H 2 from glucose at maximal level when growing at 34℃ and initial pH in 7 0 When the glucose concentration reduced to 0 4%, the hydrogen yields was increased to 1 41 mol H 2/mol glucose. Strain L15 also produced hydrogen from following carbohydrates: fructose (1 00mol H 2/mol), maltose (2 17mol H 2/mol), sucrose (1 69mol H 2/mol), inulin (4 70mol H 2/mol), glycogen (5 49mmol H 2/g) and starch (7 34mmol H 2/g).

关 键 词：氢 细菌 鉴定 产氢特性 生理特性 

分 类 号：X172[环境科学与工程—环境科学]