一株多菌灵降解细菌的分离、鉴定及系统发育分析  

Isolation, Identification and Phylogenetic Analysis of Carbendazim-degrading Bacterium Strain

作　　者：张桂山[1] 贾小明[1] 马晓航[1] 周宏斌[1,2] 程天凡[1] 

机构地区：[1]浙江大学生命科学学院 [2]广州出入境检验检疫局广州510623

出　　处：《微生物学报》2004年第4期417-421,共5页Acta Microbiologica Sinica

基　　金：国家科技部社会公益研究专项资金项目 ( 177 2 2 )~~

摘　　要：从被多菌灵污染的湖南红壤土中分离到一株能以多菌灵为唯一碳源和能源生长的菌株 1_1 ,该菌在含酵母膏多菌灵 (5 0 0mg L)的无机盐培养基中与无酵母膏的多菌灵 (5 0 0mg L)无机盐培养基中 ,2 4d对多菌灵的降解率分别为 95 5 6 %和 1 9 1 6 %。根据表型特征、G +C含量及 1 6SrDNA序列分析将菌株 1_1鉴定为 β Proteobacteria中的Ral stoniasp .(罗尔斯通氏菌 )。A bacterial strain 1_1 capable of utilizing carbendazim as the sole carbon and energy sources was isolated from carbendazim contaminated Hunan red soil. The degradation rates of carbendazim by strain 1_1 were 19 16% and 95 56% in the carbendazim (500mg/L) mineral medium and in the carbendazim (500mg/L) mineral medium supplemented with yeast extract (150mg/L) within 24d, respectively. Strain 1 1 was identified as β proteobacteria; Ralstonia sp. based on the results of phenotypic features, G+C mol% and phylogenetic analysis of 16S rDNA sequences.

关 键 词：多菌灵 生物降解 16S RDNA 罗尔斯通氏菌 系统发育 鉴定 分离 

分 类 号：X172[环境科学与工程—环境科学]