酵母蛋白多糖的分离纯化及鉴定  

Purification and Analysis of Yeast Polysaccharides

作　　者：王义华[1,2] 徐梅珍[3] 江萍[4] 何照范[4] 熊绿芸[4] 

机构地区：[1]清华大学生物科学与技术系 [2]江西农业大学食品科学系南昌330045 [3]江西农业大学食品科学系 [4]贵州大学生物技术学院

出　　处：《微生物学报》2004年第4期515-518,共4页Acta Microbiologica Sinica

基　　金：江西省自然科学青年基金资助 ( 0 3 3 0 0 61)~~

摘　　要：以热带假丝酵母和八孢裂殖酵母为材料 ,通过化学抽提法得到多糖粗取物 ,经DEAE 纤维素阴离子交换柱分离纯化得到组分Ma和Mb ,经SephadexG 2 0 0柱层析鉴定 ,它们为均一成分 ,分子量分别为 4 38kD和 6 6 7kD ;蛋白分析表明Ma和Mb为蛋白多糖 ;经薄层层析和高压液相分析 ,Ma主要含甘露糖 ,Mb则由甘露糖和半乳糖组成 ,其摩尔比为 1 3∶1 ;氨基酸组成分析表明Ma和Mb含有 4种极性氨基酸和 1 2种非极性氨基酸 ;经邻苯三酚自氧化法检测Ma与Mb均能抑制邻苯三酚自氧化 ,具有体外抗氧化能力。动物实验表明Ma和Mb具有抑制小白鼠肝癌H2 2实体瘤的作用。Two soluble fractions of Ma and Mb were purified from Candida tropicalis and Sachizosaccharomyces octosporus by ion exchanging chromatography on DEAE cellulose columns, and were proved to be homogenous with approximate molecular weights of 438kD and 667kD respectively by chromatography on Sephadex G 200 column. Protein determination and animo acid analysis showed that Ma and Mb consisted of saccharide and peptide with 4 varieties of polar and 12 of apolar animo acids; And detections by HPLC and TLC indicated that Ma was mainly composed of mannase and Mb was mainly composed of mannase and galactose in a molar ratio of 1 3∶1; Ma and Mb showed anti oxidative properties by the pyrogallol auto oxidation analysis and had inhibitory effects on the mice H22 tumor.

关 键 词：酵母 蛋白多糖 DEAE-纤维素 分离 纯化 鉴定 

分 类 号：Q936[生物学—微生物学]