RNAi及其在肿瘤研究中的应用  

RNAi and Its Application in Tumor Study

作　　者：石智[1] 符立梧[1] 

机构地区：[1]中山大学肿瘤防治中心,广州510060

出　　处：《生物化学与生物物理进展》2004年第6期492-499,共8页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 (3 0 3 7165 9)~~

摘　　要：RNA干扰 (RNAinterference ,RNAi)是指在生物体细胞内 ,外源性或内源性的双链RNA (double strandedRNA ,dsRNA)引起与其同源mRNA特异性的降解 ,因而抑制其相应的基因表达过程 .由于它能够高度特异性、高效性地抑制基因的表达 ,因此在研究基因功能及表达调控、信号传导通路、药物靶点的鉴定和基因药物开发等方面具有良好的应用前景 .主要介绍RNAi可能的分子机制、分子生物学特性。RNA interference (RNAi) is the process that the introduction of exogenous or endogenous double-stranded RNA (dsRNA) into a cell leads to special degradation of homogenous mRNA and suppression of relative gene expression. Because of being able to highly specially and efficiently silence gene expression, RNAI has the potent application in the study of gene function and regulation, signaling pathway, drug target validation and gene drug development. RNAi possible molecular mechanism, molecular biological characteristics, productive ways and its application in studying tumor are summarized.

关 键 词：RNAI SIRNA 基因抑制 肿瘤 

分 类 号：R73[医药卫生—肿瘤]