儿童急性白血病骨髓端粒酶活性异常的临床意义  

Telomerase activity and clinical characteristics of acute leukeimia in children

作　　者：申建宾[1] 汤静燕[2] 潘慈[2] 陈静[2] 薛惠良[2] 王耀平[2] 

机构地区：[1]上海第二医科大学新华医院血液科,上海200092 [2]上海儿童医学中心血液科

出　　处：《上海第二医科大学学报》2004年第5期340-342,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市高等学校科学技术发展基金(98818);上海市卫生局百人计划(BR036)资助项目

摘　　要：目的 研究儿童急性白血病(AL)骨髓端粒酶活性变化特点,探讨端粒酶活性异常的临床意义。方法 用TRAPEZETM试剂盒测定AL患儿骨髓单个核细胞端粒酶活性,分析端粒酶活性水平异常与临床指标间的相关性。结果 儿童AL骨髓端粒酶活性较正常对照高6倍;存在细胞遗传学异常者骨髓端粒酶活性高于无细胞遗传学异常者(p<0.05);初发急性非淋巴细胞白血病(ANLL)中骨髓端粒酶活性与发病时外周血白细胞计数呈显著线性正相关(r=0.70,P<0.05);ANLL中,高端粒酶活性组出现复发或死亡的机率高于低活性组;AL完全缓解时骨髓端粒酶活性降至正常范围,复发时骨髓端粒酶活性再度升高。结论 儿童AL时骨髓端粒酶活性升高,完全缓解时降至正常;端粒酶活性与AL诸临床指标的相关性提示其作为AL危险因素的可能性。Objective To investigate the characteristics and clinical significance of telomerase activity (TA) in childhood acute leukemia (AL). Methods TA in mononuclear cells separated from bone marrow (BM) of leukemia patients was determined with the TRAPEZE?kit and expressed as total product generated. The interrelated data deriving from laboratory and clinical material were analyzed with SPSS 10. 0 statistical software. Results The median TA in AL was 6-fold higher than that in normal control. TA in AL with cytogenetic abnormality was higher than AL with normal cytogenetics( P <0. 05). There existed a positive correlation in ANLL between TA and total leukocytes count of peripheral blood at the time of ANLL onset (r = 0. 70, P < 0. 05). TA in AL drop into normal range when patients reached complete rimission, while TA increased again when AL relapsed. Conclusion TA in AL increased dramatically at the initial presentation, dropped into normal range when AL complete rimission. Our research has raised the possibility of TA as a risk factor in pediatric AL.

关 键 词：儿童 急性白血病 端粒酶 活性检测 临床指标 病因分析 

分 类 号：R733.71[医药卫生—肿瘤]