尿液中多环芳烃羟基代谢产物分析方法研究  

Analysis of Hydroxyl Polycyclic Aromatic Hydrocarbons in Human Urine

作　　者：段小丽[1] 杨洪彪[2] 张林 张军锋 许军[1] 赵淑莉[4] 魏复盛[4] 

机构地区：[1]北京科技大学,北京100083 [2]鞍山市环境监测中心,辽宁鞍山114000 [3]美国新泽西医学与牙医学大学环境与职业健康研究所,美国新泽西08854 [4]中国环境监测总站,北京100029

出　　处：《环境科学研究》2004年第3期62-65,共4页Research of Environmental Sciences

基　　金：中-美合作项目(美国癌症研究所1R-CA94743-01)

摘　　要：通过实验建立了"酶水解-固相萃取-HPLC 荧光检测"尿液中多环芳烃羟基代谢产物1-羟基芘,9-羟基苯并[a]芘和3-羟基苯并[a]芘的分析方法,研究了3种代谢产物在不同保存温度下的稳定性,并通过对200个实际尿液的测定初步验证了该方法的可行性。该方法回收率为70%～85%,最低检出限为0 02～0 05μg L。-20℃保存的尿液样品及尿液提取液中3种化合物在1个月内降解了10%～36%,提取液比尿液的稳定性稍好,保存温度越高稳定性越差。A method to measure urinary metabolites of polycyclic aromatic hydrocarbons(PAHs) was established based on experiments.The method involves enzymatic hydrolysis of PAHs metabolites and HPLC-fluorescence detection of three hydrolysis products,i.e. 1-OH-pyrene,9-OH-benzo[a] pyrene and 3-OH-benzo[a]pyrene.The stability of these metabolites under different storage temperature was also examined.The results from the analysis of 200 human urine samples preliminarily confirmed the feasibility of this method.Recovery rate of these metabolites by this method is 70%～85%.Method detection limit is 0.02～0.05 μg/L.Conentration of these three metabolites is degraded by 10%～36% when stored at -20　℃ after one month.Stability in urine extract is better than in original urine.Higher storage temperature may cause worse stability.

关 键 词：尿液 多环芳烃 代谢产物 1-羟基芘 9-羟基苯并[a]芘 3-羟基苯并[a]芘 生物监测指标 

分 类 号：X132[环境科学与工程—环境科学]