被动吸烟对大鼠脑组织iNOS表达的影响  

The Effect of Passive Smoking on the Expression of Inducible Nitric Oxide Synthase in the Brain of Rats

作　　者：孙晓红[1] 柳忠兰[2] 冯昱[2] 

机构地区：[1]沈阳铁路局中心医院神经内科,辽宁沈阳110032 [2]中国医科大学附属第一医院神经内科,辽宁沈阳110001

出　　处：《解剖科学进展》2004年第2期119-121,共3页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金 (No 9810 5 0 0 3 0 3 );卫生部科学研究基金 (No 98-1-196)

摘　　要：目的　研究被动吸烟大鼠脑组织诱发型一氧化氮合酶 (iNOS)的变化 ,探讨iNOS在吸烟导致脑损伤中的作用机制。方法　制备被动吸烟大鼠模型 ,采用免疫组织化学ABC方法检测脑组织iNOS表达 ,应用光镜、透射电镜观察海马神经元的病理改变。结果　正常对照组大鼠大脑皮层、海马、纹状体均有少量iN OS表达 ,短期吸烟组与正常对照组比较有显著差异 (P <0 0 1) ,长期小量或大量吸烟组 (3个月 )各脑区iN OS表达显著高于正常对照组和短期吸烟组 (P<0 0 1)。长期大量吸烟组可见海马神经元变性改变。结论　吸烟可使脑组织iNOS活化表达增强生成过多的NO造成脑神经细胞毒性损伤 ,存在量效、时效关系。Objective To study the effect of passive smoking on the expression of inducible nitric oxide synthase in the brain of rats. Methods ABC immunohistochemical method was used to study the expression of iNOS and pathologic change of neuron was observed under transmission electron microscope . Results Expression of iNOS in cerebral cortex, hippocampus and corpus striatum is increased in short-term passive smoking group (P<0.05) and increased significantly in long-term passive smoking group (P<0.01) compared with control group and also the degenerated neurons were observed sometimes in long-term passive smoking group.Conclusion Cigarette smoking cause cytotoxic lesion through synthesis of NO via increased expression of iNOS in brain,with dose-effect and time-effect relationships between cigarette quantity or time and the lesion.

关 键 词：被动吸烟 大鼠 脑组织 INOS 诱发型一氧化氮合酶 脑血管疾病 

分 类 号：R743[医药卫生—神经病学与精神病学]