自体外周血干细胞移植治疗重症肌无力的造血干细胞动员和采集初步研究  

Elementary study of mobilization and harvesting after autologous peripheral blood stem cell transplantation on myasthenia gravis

作　　者：秦超[1] 莫雪安[1] 凌莉[1] 赖永榕[2] 孙圣刚[3] 童萼塘[3] 

机构地区：[1]广西医科大学第一临床医学院神经内科,南宁530021 [2]广西医科大学第一临床医学院血液科,南宁530021 [3]华中科技大学同济医学院协和医院神经内科

出　　处：《临床神经病学杂志》2004年第3期178-180,共3页Journal of Clinical Neurology

基　　金：广西省科技攻关项目资助 (桂科攻 0 32 2 0 2 5 9)

摘　　要：目的　探讨外周血干细胞移植 (PBSCT)治疗重症肌无力 (MG)的造血干细胞动员和采集的有效性。方法　MG患者 1例 ,用环磷酰胺 (CTX) +粒细胞集落刺激因子 (G CSF)联合动员 ,用细胞分离机采集外周血干细胞 ;用流式细胞测定动员后CD+ 3 4 细胞数和用体外半固体甲基纤维素培养法测定单核细胞集落形成单位 (GM CFU)数 ;用相对评分法评估造血干细胞移植后患者的临床疗效。结果　动员后单核细胞 (MNC)为8 0 8× 10 8/kg、GM CFU数为 7× 10 5/kg ,或集落数为 81× 10 5/MNC和CD+ 3 4 细胞数为 1 0 9× 10 6/kg。相对评分法患者的临床疗效为 7/ 8分。结论　CTX +G CSF能有效地动员外周血干细胞 ,可以为移植提供足够数量的造血干细胞数。Objective To explore the clinical effect of mobilization and harvesting by autologous peripheral blood stem cell transplantation (APBSCT) on treating myasthenia gravis (MG).Methods One patient with MG was enrolled in the study. Autologous peripheral blood stem cells(APBSCs) were mobilized with cyclophosphamide(CTX) and granulocyte colony-stimulating factor(G-CSF). PBSCs harvesting was performed by continuous flow leukapheresis using a CS-3000 plus blood separator.CD +_ 34 cells were analyzed by flow cytometer and granulocyte-macrophage progenitors (GM-CFU) was detected by semisolid methlcelluose after mobilization. The clinical effect was evaluated by a relative score system.Results The numbers of mononuclear cells (MNC),GM-CFU and CD +_ 34 cells were 8.08×10 8/kg,7×10 5/kg or 81/10 5/MNC and 1.09×10 6/kg after mobilization in graft respectively. The clinic relative score of the patient was 7/8.Conclusion CTX and G-CSF can mobilize effectively and provide enough peripheral blood stem cells for transplantation in treating MG.

关 键 词：重症肌无力 外周血干细胞移植 动员和采集 

分 类 号：R746[医药卫生—神经病学与精神病学]