尼膜同对大鼠脑缺血再灌注后血-脑屏障通透性和脑梗死体积的影响  

The effects of Nimotop on the permeability of blood-brain barrier and cerebral infarction size after cerebral ischemic reperfusion in rats

作　　者：任丽[1] 孙善全[1] 

机构地区：[1]重庆医科大学神经生物学研究室,400016

出　　处：《临床神经病学杂志》2004年第3期187-190,共4页Journal of Clinical Neurology

基　　金：国家自然科学基金资助课题 (NO :30 0 70 2 4 7)

摘　　要：目的　研究尼膜同对大鼠脑缺血再灌注后血 脑屏障 (BBB)通透性和脑梗死体积的影响。方法采用插线法制作脑缺血再灌注的大鼠模型 ,缺血 2h后再灌注。实验分尼膜同组和生理盐水对照组 ,每组分再灌注 6h、12h、2 4h、4 8h、72h 5个时段 ,再灌注后立即分别腹腔注射尼膜同 2mg/kg或等量的生理盐水 ,每12h注射 1次。用荧光法及透射电镜观察不同时段BBB通透性破坏的情况 ,TTC染色后计算梗死体积百分比。结果　脑缺血再灌注后随时间延长 ,BBB通透性和梗死体积百分比逐渐增加 ,且BBB通透性的增加呈现两个高峰 ,分别为再灌注后 12h和 4 8h ;尼膜同组BBB通透性及脑梗死体积百分比明显高于生理盐水对照组 ,差异有显著性 (P <0 0 5 )。结论　脑缺血再灌注增加BBB的通透性和脑梗死体积百分比 ,再灌注后给予尼膜同可加重这些病理变化。Objective To study the effect of Nimotop on the permeability of blood-brain barrier (BBB) and cerebral infarction size after cerebral ischemic reperfusion (CIR) in rats.Methods CIR models were established by occluding unilateral middle cerebral arteries(MCA) of rats for 2 hours and then reperfused. The models were divided into 2 groups:Nimotop group and the saline control group,each group had CIR 6、12、24、48 and 72 h sub-groups. As soon as reperfusion,Nimotop or saline was abdominal abministrated(2 mg/kg weight) respectively per 12 h. Fluorophotometry and transmission electron microscopy were used to detect the permeability of BBB,the rate of infarction size was calculated by 2,3,5-triphenyl tetrazolium chloride(TTC) dyeing.Results Following reperfusion,the permeability of BBB and the rate of cerebral infarction size were increased. The permeability of BBB had two peaks. The first peak took place at 12h after CIR. The second peak was at 48 h after CIR. These changes were more severe in Nimotop group than in saline group( P < 0.05).Conclusion CIR destroyed the integrality of BBB and increased the size of cerebral infarction. Use of Nimotop may aggravate these pathological changes after reperfusion.

关 键 词：脑缺血再灌注 血-脑屏障 脑梗死体积 尼膜同 

分 类 号：R743.3[医药卫生—神经病学与精神病学]