格列卫与六亚甲基二乙酰胺对K562细胞增殖和凋亡的影响  

Effects of Glivec and hexamethylenebisacetamide on proliferation and apoptosis of K562 cells

作　　者：王玮[1] 孙秉中[1] 刘新平[2] 冯琦[1] 尚振川[1] 曹云新[3] 药立波[2] 

机构地区：[1]第四军医大学西京医院血液科,陕西西安710033 [2]第四军医大学基础部生物化学与分子生物学教研室,陕西西安710033 [3]第四军医大学础部免疫学教研室,陕西西安710033

出　　处：《第四军医大学学报》2004年第12期1069-1072,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 (39770 336) ;陕西省科研基金资助项目 (98SM40 )

摘　　要：目的 :研究格列卫和六亚甲基二乙酰胺 (HMBA)对K5 6 2细胞增殖、凋亡、细胞周期及周期蛋白表达的影响 .方法 :MTT法观察细胞生长指数和细胞存活率 ,流式细胞仪、电镜检测细胞周期和凋亡 ,半定量RT PCR检测bcr abl基因的表达 ,Westernblotting检测蛋白表达 .结果 :联合应用两种药物后细胞存活率明显下降 ,凋亡比例略下降 ,细胞周期阻滞于G0 /G1 期 ,cyclinD1、cyclinE、cyclinA、CDK4、c myc蛋白表达下调 .结论 :两种药物应用后出现细胞存活率下降、细胞周期阻滞、周期蛋白表达下调、凋亡比例下降 ,联合作用对细胞的增殖抑制具有协同作用 。AIM: To explore how Glivec and HMBA affect the cell growth, apoptosis, cell cycle and cyclin protein expression of K562 cells. METHODS: After K562 cell line was cultured and Glivec and/or HMBA were added, the cell growth index and surviving rate were determined with MTT assay, the cell cycle and apoptosis were examined by flow cytometry and electron microscopy, the expression of bcr abl gene was examined by RT PCR, and the expressions of cyclinD1, cyclinE, cyclinA, CDK4 and c myc protein were examined by Western blot. RESULTS: A combination of two drugs apparently reduced the surviving rate and slightly declined the percent of apoptosis. K562 cells were arrested in G0/G1 phase, and the expressions of cyclinD1, cyclinE, cyclinA, CDK4 and c myc protein were downregulated. CONCLUSION: A combination of two drugs can effectively inhibit the growth and slightly inhibit the apoptosis of K562 cells. The action of drugs bears on cell cycle arrest, and downregulation of cyclin protein and cell apoptosis.

关 键 词：格列卫 六亚甲基二乙酰胺 增殖抑制 细胞周期 脱噬作用 

分 类 号：R73-35[医药卫生—肿瘤]