釉基质蛋白体外诱导大鼠外胚间充质细胞骨桥素和骨钙素mRNA的表达  

Expression of Osteopontin and Osteocalcin mRNA in Ectomesenchymal Stem Cells under Induction of EMPs & EMD in Vitro.

作　　者：柴枫[1] 金岩[1] 赵铱民[1] 段小燕[1] 

机构地区：[1]第四军医大学口腔医学院修复科,陕西西安710032

出　　处：《口腔医学研究》2004年第3期243-245,共3页Journal of Oral Science Research

基　　金：第四军医大学创新工程重点课题 (编号 :CX0 2F0 0 2 )

摘　　要：目的 :了解体外培养环境中 ,大鼠外胚间充质细胞在两种不同形式釉基质蛋白 (粗提型EMPs及纯化型EMD)诱导条件下 ,成骨分化标志骨桥素及骨钙素mRNA的表达水平。方法 :酶消化法原代培养SD大鼠颌突外胚间充质细胞 ,在含釉基质蛋白 (EMPs或EMD或EMPs +EMD)的培养液中进行体外诱导培养 ,提取总RNA ,采用RT -PCR法 ,以β -actincDNA为内参照 ,检测骨桥素及骨钙素mRNA的表达水平。结果 :1)各诱导实验组细胞均出现骨桥素mRNA的表达 ;2 )仅EMPs+EMD组出现骨钙素mRNA的表达。结论 :外胚间充质细胞经釉基质蛋白特别是粗提型EMPs及纯化型EMD体外复合和诱导培养 ,可向成骨或成牙骨质方向分化 。Objective: Ectomesenchymal stem cells (EMSCs) are considered to be able to differentiate toward a cementoblast/osteoblast phenotype. This study is to investigate Osteopontin and Osteocalcin mRNA expression of Rat ectomesenchymal stem cells under inducing condition of EMPs & EMD in vitro. Methods: Cultured EMSCs were exposed to the EMPs- or EMD- or (EMPs+EMD)- conditioned culture media. Osteoblast MC3T3-E1 served as positive control (P) and non-induced EMPs as negative control (N). Then Osteopontin and Osteocalcin mRNA expression were measured by RT-PCR technique. Result: 1) There was expression of Osteopontin mRNA in group EMPs, group EMD and group (EMPs+EMD) after induction for 3 weeks ; 2) There was expression of Osteocalcin mRNA only observed in group (EMPs+EMD). Conclusion: The cultured Rat EMSCs kept non-osteogenic differentiated state by itself; but may have osteogenic or cementogenic potential testified by expression of Osteopontin and Osteocalcin mRNA when induced by EMPs.

关 键 词：釉基质蛋白 外胚间充质细胞 骨桥素mRNA 骨钙素mRNA 

分 类 号：R783[医药卫生—口腔医学]