转染外源HCAP1基因对Raji细胞化疗敏感性的作用  

Effects on Sensitivity to Chemotherapy in the Raji Cell Transfected with Exogenous HCAP1 Gene

作　　者：杜均祥[1] 王嵘[1] 吴向华[1] 万大方[2] 顾建人[2] 

机构地区：[1]暨南大学医学院血液病研究所 [2]上海市肿瘤研究所癌基因与相关基因国家重点实验室

出　　处：《肿瘤防治研究》2004年第6期330-332,共3页Cancer Research on Prevention and Treatment

基　　金：广东省医学科研基金 (A2 0 0 130 9);广东省自然科学基金 (No .0 10 4 11)资助项目

摘　　要：目的　探讨外源HCAP1基因产物对B淋巴瘤 /白血病细胞系Raji细胞化疗敏感性的作用。 方法　用脂质体介导法 ,把HCAP1基因转染Raji细胞 ,经G4 18筛选后 ,分别与化疗药物DNR(daunoru bicin ,柔红霉素 )、VP16 (etoposide ,足叶乙甙 )和VCR(vincristine,长春新碱 )联合培养 2 4小时 ,用苔盼兰拒染试验计数活细胞。结果　转染HCAP1的Raji细胞 ,在不同浓度的DNR(0 .0 1～ 10 .0 μg/ml)和VP16 (5～ 80 μg/ml)作用下 ,与转染空载体和未转染对照细胞比较 ,细胞活力的抑制作用明显增强 (P <0 .0 5 )、IC50 (半数抑制浓度 )值明显降低 ;但与不同浓度的VCR(12 .5～ 10 0 μg/ml)作用 ,细胞活力的抑制和IC50 值与对照细胞相似。结论　HCAP1过表达可明显增加Raji细胞对DNR、VP16作用的敏感性 。Objective To investigate the effects of exogenous HCAP1 gene product on the sensitivity to chemotherapy in B lymphocytic lymphoma/leukemia cell line Raji.Methods HCAP1 gene was transfected into Raji cells by medium with liposome, The Raji cells stably expressing exogenous HCAP1 gene, which were screened by G418, exposed to various concentration of DNR, VP16 and VCR for 24 hours, the cell viability was measured by trypan blue dye exclusion test.Results Following exposure to various concentrations of DNR( 0.01 ～ 10.0μg/ml )and VP16(5～80μg/ml), Raji cell tranfected with HCAP1, displayed stronger suppression on cell viability and lower level of IC 50 value, compared with the control cells transfected with empty vector or without any transfection. However, similar suppression on cell viability and IC 50 value were observed between Raji cells transfected with HCAP1 and control cells, following exposure to VCR( 12.5 ～100μg/ml).Conclusion Overexpreesion of HCAP1 could increase sensitivity in the Raji cells to DNR and VP16, but not to VCR.

关 键 词：HCAP1基因 基因转染 细胞活力 RAJI细胞 

分 类 号：R733.73[医药卫生—肿瘤]