B-ALL病人TCR Vβ基因谱系和克隆性分析  

Analysis of T-cell Receptor Vβ Gene Repertoire and Clonality in Patients With B-ALL

作　　者：黄梅娟[1] 李扬秋[1] 陈少华[1] 杨力建[1] 周羽竝[2] 张涛[1] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632 [2]暨南大学医学院生化教研室,广州510632

出　　处：《肿瘤防治研究》2004年第6期320-322,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(39870 35 8);中德生物技术合作项目(CHN0 2/319);广东省自然科学基金(980 70 5 );广东省科技计划项目(2 0 0 2C10 4 0 5 0 1);广州市科技攻关计划 (98 z 0 39 0 1;2 0 0 3J1 I0 0 11)

摘　　要：目的　了解B ALL病人外周血T细胞的TCRVβ基因谱系及其克隆性增殖情况。 方法　利用RT PCR方法扩增 13例初发未治B ALL病人外周血单个核细胞中 2 4个TCRVβ基因的互补决定区 3(CDR3) ,PCR产物进一步经荧光标记和基因扫描分析CDR3长度而确定T细胞的克隆性。结果　 13例B ALL病人分别表达 2～ 18个Vβ亚家族。 13例病人均存在 1个或多个Vβ亚家族的克隆增殖T细胞 ,增殖形式包括寡克隆及寡克隆增殖趋势、双克隆和单克隆。Vβ2 1和Vβ2 3亚家族出现克隆增殖性T细胞的频率较高。结论　B ALL病人外周血T细胞的TCRVβ谱系呈现优势利用的特点 ,并存在克隆性增殖的T细胞 ,这可能是机体对白血病相关抗原产生的特异性免疫反应 ;Vβ2 1和Vβ2 3亚家族T细胞发生克隆增殖改变的趋向性比较明显 ,可能与BObjective To investigate the situation of TCR Vβ gene repertoire and clonal expansion in peripheral blood T cells from patients with B ALL.Methods The complementarity determining region 3 (CDR3) of TCR Vβ 24 subfamily genes in peripheral blood mononuclear cell from 13 cases with untreated B ALL were amplified using RT PCR. The PCR products were further labelled by fluorescein and then analyzed by genescan technique for detection of the CDR3 size, to determine clonality of T cells.Results 2～18 Vβ subfamilies were identified in 13 B ALL cases respectively. Clonal expansive T cells in one or more Vβ subfamilies were found in all 13 cases, including oligoclonal/oligoclonal trend, bioclonal and monoclonal patterns. The display frequency of clonal expansive T cells in Vβ21 and Vβ23 subfamilies were higher than others.Conclusion The dominant utilization of TCR Vβ repertoire could be found in peripheral blood T cells from patients with B ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia associated antigen. The clonal expansive tendency of T cells in Vβ21 and Vβ23 subfamilies was rather obvious, which maybe correlated with certain malignant B cell clone.

关 键 词：B细胞-急性淋巴细胞白血病 T细胞受体VΒ基因 T细胞克隆性 

分 类 号：R733.73[医药卫生—肿瘤] R371.22[医药卫生—临床医学]