膀胱移行细胞癌中bax、bcl-2、CD44v6、nm23表达  

Expression of bax、bcl-2、CD44v6、nm23 protein in transitional cell carcinoma of the bladder

作　　者：张玉华[1] 陈萍[1] 朴颖实[1] 韩影[1] 李弘[1] 张伟东[1] 刘宪军[1] 巩雷[1] 谢江[1] 

机构地区：[1]吉林市中心医院病理科,吉林132011

出　　处：《临床与实验病理学杂志》2004年第3期320-323,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的　探讨bax、bcl 2、CD4 4、nm2 3基因蛋白在膀胱移行细胞癌 (TCC)中的表达。方法　应用免疫组化S P法对 6 4例膀胱TCC及 2 0例正常膀胱黏膜组织中bax、bcl 2、CD4 4v6、nm2 3进行检测。结果　 6 4例膀胱TCC中bax阳性率为 17 2 % (11/6 4 ) ,正常膀胱黏膜组织为 90 0 % (18/ 2 0 ) (P <0 0 5 )。膀胱TCC中bcl 2阳性表达率为 82 8% (5 3/ 6 4 ) ,正常膀胱黏膜为 2 0 0 %(4 / 2 0 ) (P <0 0 5 )。bax、bcl 2阳性率随组织学分级的提高有逐渐增强的趋势 ,但无统计学意义 (P >0 0 5 ) ;在无浸润及有深层浸润的膀胱TCC中 ,bax的阳性表达率分别为 16 3%、2 0 0 % (P >0 0 5 ) ,bcl 2则分别为 83 7%、80 0 % (P >0 0 5 ) ;在无复发转移及有复发转移的膀胱TCC中 ,bax阳性率分别为 16 1%、2 5 0 % (P >0 0 5 ) ,bcl 2分别为 82 1%、87 5 % (P >0 0 5 )。 6 4例膀胱TCC中CD4 4v6阳性表达率为 5 0 0 % (32 / 6 4 ) ,正常膀胱黏膜组织为 5 0 % (1/ 2 0 ) (P <0 0 5 )。nm2 3在膀胱TCC中的阳性率为 76 6 % (4 9/ 6 4 ) ,正常膀胱黏膜组织为 2 0 0 % (4 / 2 0 ) (P <0 0 5 )。CD4 4v6阳性率Ⅰ、Ⅱ级与Ⅲ级相比差异有显著性 (P <0 0 5 ) ,nm2 3阳性率Ⅰ级与Ⅱ、Ⅲ级相比差异有显著性 (P <0Purpose To study the expression of bax,bcl-2,CD44v6,nm 23 protein in transitional cell carcinoma(TCC) of the bladder.Methods S-P immunohistochemical staining was performed to evaluate the positivity of bax,bcl-2,CD44v6 ,nm23 in 64 cases of TCC and 20 normal bladder mucosa. Results The positive rate of bax was 17.2% (11/64) in 64 cases of TCC, but 90% (18/20) in 20 normal bladder mucosa(P<0.05). For bcl-2, it’s expression were 82.8% (53/64) and 20.0% (4/20) respectively (P<0.05). The positive rate of bax and bcl-2 had the trend of improvement with the development of grade, but it hadn’t the positive correlation (P>0.05). In non-invasions and depth-invasions of TCC,bax expression was 16.3% and 20.0% respectively (P>0.05),bcl-2 expression was 83.7% and 80.0% respectively (P>0.05). In non-recurrences or non-metastases and in recurrences or metastases of TCC, bax expression was 16.1% and 25.0% respectively (P>0.05), bcl-2 expression was 82.1% and 87.5% respectively (P>0.05). In 64 cases of TCC,CD44v6 expression was 50.0% (32/64) but in normal bladder mucosa it was 5.0% (1/20) (P<0.05). For nm23, it’s expression was 76.6% (49/64) and 20.0% (4/20) respectively (P<0.05).The expression of CD44v6 protein in grade Ⅰ ,Ⅱ and grade Ⅲ had remarkable distinction (P<0.05) and nm23 in grade Ⅰ and grade Ⅱ,Ⅲ had remarkable distinction (P<0.05).The expression of CD44v6,nm 23 in invasions had higher level than non-invasions.CD44v6 expression in metastases had higher level than non-metastases (P<0.05),while nm23 expression hadn’t relation to its metastases (P>0.05).Conclusions The decrease of bax protein and the increase of bcl-2 protein playedcon important role in occurence of bladder TCC.CD44v6, nm23 protein also promoted the tumors’ occurrence;bax and bcl-2 protein handn’t relation to the tumors’ grade,depth-invasion,recurrence or metastasis;CD44v6 expression had positive relation to the grade,depth-invasion,recurrence or metastasis;nm23 had positive relation to the grade,depth-invasion,but hadn

关 键 词：膀胱移行细胞癌 BAX BEL-2 CD44V6 NM23 免疫组织化学 

分 类 号：R737.14[医药卫生—肿瘤]