丹皮酚对大鼠脑缺血再灌注后细胞间黏附分子-1表达的影响  

Effect of Paeonol on intercellular adhesion molecule 1 expression after cerebral ischemia reperfusion in rats

作　　者：孙文芳[1] 王朝晖[2] 高金霞[3] 臧玉华[4] 

机构地区：[1]大连医科大学附属第二医院神经内科,辽宁省大连市116027 [2]大连医科大学附属第二医院实验中心,辽宁省大连市116027 [3]大连医科大学附属第二医院麻醉科,辽宁省大连市116027 [4]大连医科大学附属第二医院病理科,辽宁省大连市116027

出　　处：《中国临床康复》2004年第19期3792-3793,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察丹皮酚对大鼠脑缺血再灌注后细胞间黏附分子-1(intercellularadhesionmolecule-1,ICAM-1)蛋白表达的影响。方法:利用线栓法制备大鼠大脑中动脉缺血2h再灌注24h模型,治疗组缺血时腹腔注射丹皮酚。行苏木精-伊红染色计数缺血区中性粒细胞浸润及坏死神经元的数目,应用免疫组化方法检测ICAM-1蛋白的表达情况。结果:丹皮酚治疗组坏死神经元百分率犤(25.85±3.93)%犦低于对照组犤(31.13±4.58)%,t=2.770,P<0.05犦。中性粒细胞的浸润数治疗组犤(15.40±2.59)个/视野犦较对照组犤(19.10±2.81)个/视野犦显著减少(t=3.063,P<0.01)。ICAM-1的表达治疗组犤(13.90±2.18)条/视野犦较对照组犤(16.20±2.30)条/视野犦下调(t=2.290,P<0.05)。结论:丹皮酚可能具有抑制大鼠脑缺血再灌注后ICAM-1蛋白表达的作用,从而减轻了神经元损伤。AIM:To investigate the effect of Paeonol on the expression of intercellular adhesion molecule 1(ICAM 1) protein after focal cerebral ischemia reperfusion in rats. METHODS:Rat models of middle cerebral artery occlusion were established with thread after 2 hour ischemia and 24 hour reperfusion.Intraperitoneal injection of Paeonol was performed in the treatment group.The number of neutrophil infiltration and necrotic neurons in ischemic areas was counted with hematoxylin eosin staining and the expression of ICAM 1 protein was tested by immunohistochemistry. RESULTS:The percentage of neuronal damage was decreased in the treatment group[(25.85±3.93)%] in comparison with the control group[(31.13±4.58)%,t=2.770,P< 0.05] .The number of neutrophil infiltration in the treatment group[(15.40±2.59)per visual field] was sighificantly lower than that in the control group[(19.10±2.81)per visual field,t=3.063,P< 0.01].A decrease in the ICAM 1 expression was detected in the treatment group[(13.90±2.18)per visual field]as compared with the control group[(16.20±2.30)per visual field](t=2.290,P< 0.05). CONCLUSION:Paeonol may inhibit ICAM 1 protein expression after focal cerebral ischemia reperfusion in rats so as to relieve neuron damage.

关 键 词：丹皮酚 大鼠 脑缺血 再灌注损伤 细胞间黏附分子-1 腹腔注射 

分 类 号：R743.31[医药卫生—神经病学与精神病学]