神经肽Y剌激肾动脉平滑肌热休克蛋白表达的实验研究  

Stimulation of neuropeptide Y on heat shock protein expression in renal vascular smooth muscle

作　　者：何慧婵[1] 钟惟德[1] 曾广翘[1] 刘建康[2] 黄少华[3] 陈敏生[3] 魏鸿蔼[1] 

机构地区：[1]广东省广州市第一人民医院泌尿外科,510180 [2]广州医学院组织胚胎学教研室 [3]广州医学院附属第二医院心内科

出　　处：《中华实验外科杂志》2004年第5期571-572,共2页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金资助项目 (980 4 66) ;广州市科委基础研究资助项目 (99 J 0 0 9 0 1 )

摘　　要：目的　以热休克蛋白 70 (HSP 70 )作为神经肽Y (NPY )刺激肾动脉平滑肌细胞增殖活动为信号 ,探讨高血压肾动脉重塑中发挥的作用。方法　应用快速微量MTT比色法和荧光免疫组织化学定量技术观察神经肽Y作用下肾动脉平滑肌细胞 (VSCM )增殖活动中HSP70表达作用。结果　神经肽Y可刺激体外培养肾动脉平滑肌细胞的增殖 ,其增殖度 (MTT A值 )和细胞内因损伤产生的HSP 70表达明显高于对照组 (P <0 .0 5 )。结论　神经肽Y可影响肾动脉平滑肌细胞增殖 ,而HSP 70表达水平是肾动脉平滑肌细胞增殖的重要信号。Objective To study the effect of neuropeptide Y (NPY) on renal arteries in hypertension by detecting heat shock protein (HSP70),a signal of proliferation of the renal vascular smooth muscle.Methods By using automated MTT colorimetric microassay and quantitative immunocytochemistry technique,the expression of HSP70 in renal vascular smooth muscle cell (VSMC) proliferation subjected to NPY stimulation was observed.Results NPY could stimulate the proliferation of renal VSMC in vivo.The MTT-A of proliferation and expression of intracellular injury-generated HSP70 were significantly higher under NPY stimulation than in controls ( P <0.01).Conclusion NPY can influence the renal VSMC proliferation and HSP70 is a signal of proliferation of the renal vascular smooth muscle.

关 键 词：神经肽Y 剌激 肾动脉平滑肌 热休克蛋白 表达 实验研究 免疫组织化学 

分 类 号：R363[医药卫生—病理学]