七叶灵方诱导裸鼠人肺腺癌A549移植瘤细胞凋亡的实验研究  

Experimental study of Qiyeling Decoction inducing apoptosis of transplanted human lung adenocarcinoma cells A549 in nude mice

作　　者：金长娟[1] 沙慧芳[2] 赵兰香[2] 冯久贤[2] 顾伟勇[2] 史振余[2] 

机构地区：[1]上海市胸科医院中西医结合科,上海200030 [2]上海市胸科医院肿瘤研究室,上海200030

出　　处：《中西医结合学报》2004年第4期285-287,共3页Journal of Chinese Integrative Medicine

基　　金：上海市综合性医院中医科达标创建单位基金资助项目(No .沪卫中医 2 0 0 3 19)

摘　　要：目的 :研究中药复方七叶灵方诱导人肺腺癌A5 4 9细胞裸鼠移植瘤细胞凋亡的作用。方法 :　皮下接种人肺腺癌A5 4 9细胞 ,建立裸鼠移植瘤模型 ,随机分为不用任何治疗的对照组 (A组 )、化疗组 (B组 )、化疗加七叶灵治疗组 (C组 )和七叶灵治疗组 (D组 ) ,每组 12只 ,分别给予相应处理。治疗开始后每周测量肿瘤体积并换算成瘤重。采用原位细胞凋亡检测试剂盒 ,检测瘤组织凋亡细胞数。结果 :A组瘤重显著高于其他各组 (P <0 .0 5 ) ,C组、D组与B组比较亦有显著差异 (P <0 .0 5 )。凋亡细胞数各组间具有显著差异 (P <0 .0 5 ) ,D组瘤组织中的凋亡细胞数显著高于B组和C组 (P <0 .0 5 )。A组则表现出早期细胞为高分化 ,到后期肿瘤组织坏死及部分细胞凋亡。结论 :在裸鼠体内中药七叶灵方具有诱导A5 4Objective:To study the function of Qiyeling Decoction in inducing apoptosis of transplanted human lung adenocarcinoma cells A549 in nude mice.Methods:Nude mice with transplanted A549 tumor were randomly divided into the untreated control group (group A), chemotherapy treated group (group B), chemotherapy plus Qiyeling Decoction treated group (group C), Qiyeling Decoction treated group (group D) and managed correspondingly. The tumor volume was measured and calculated into tumor weight. The apoptosis of tumor cells were examined using in situ cell apoptosis detection kit.Results:The tumor weight was lower obviously in groups B, C and D than that in group A (P<0.05). The apoptosis of tumor cells was lower obviously in groups B, and C than that in group D (P<0.05). Cells in group A appeared perfect differentiation during the early stage and apoptosis later.Conclusion:Qiyeling Decoction can induce A549 cell apoptosis in nude mice.

关 键 词：七叶灵方 裸鼠 肺腺癌 移植瘤 细胞凋亡 实验 A549细胞 中医药疗法 

分 类 号：R734.2[医药卫生—肿瘤]